168599 20260211123813.0 doi 10.1080/1828051X.2026.2622223 sideral 148006 ART-2026-148006 eng Muñoz-Grein, Jennifer Universidad de Zaragoza (orcid)0009-0005-9117-8617 In vitro effects of saponins, tannins and lipids on rumen fermentation and intestinal health markers in a ruminant weaning context 2026 Dairy ruminants often experience physiological disturbances during the post-weaning, includingsuboptimal rumen fermentation due to an underdeveloped rumen microbiota, and diarrhoeaassociated with intestinal inflammation and oxidative stress. This study explored six commercialfeed additives with a potential dual mode of action to optimise both rumen fermentation andintestinal health in a post-weaning scenario. The experimental treatments included dietary sup-plementation with saponins (SAP), tannins (TAN), lauric acid (LAU), fish oil (FIO), high-unsaturatedolein (HUO) and high-saturated olein (HSO). Additives were tested at four doses (0, 100, 300 and600 mg/L) with four experimental replicates. Effects on rumen fermentation were evaluated inbatch cultures using inocula from dairy lambs and a high concentrate substrate, while intestinalhealth responses were evaluated in Caco-2 cells. Saponins (p ¼ 0.01) and tannins (p < 0.01) lin-early decreased rumen ammonia-N concentration in batch cultures. In Caco-2 cells, saponinsimproved metabolic activity (p ¼ 0.04), whereas tannins reduced pro-inflammatory IL-6 concentra-tion (p < 0.01), suggesting beneficial effects on intestinal health. Lipid sources exerted minimaleffects on rumen fermentation and induced intestinal inflammation when supplemented at highdoses. Overall, the physiological constrains of dairy ruminants during the post-weaning period,such as an immature rumen microbiota, absence of protozoa and low rumen pH, may limit theefficacy of additives in improving rumen fermentation. Nevertheless, saponins and tanninsshowed potential to reduce rumen proteolysis and support intestinal health during this criticaldevelopmental stage. Further in vivo research is required to validate these findings. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/AEI/MICNN/PID2021-123206OB-I00 info:eu-repo/grantAgreement/ES/MICINN PRE2022-101806 info:eu-repo/grantAgreement/ES/MICINN/RYC2019-027764-I/AEI/10.13039/501100011033 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Solanas, Estela Universidad de Zaragoza (orcid)0000-0001-8056-5236 Fondevila, Manuel Universidad de Zaragoza (orcid)0000-0002-0712-1185 Belanche, Alejandro Universidad de Zaragoza (orcid)0000-0001-5880-6021 1003 027 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Anatom.Embriol.Humana 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 25, 1 (2026), 125-137 Ital. J. Anim. Sci. Italian Journal of Animal Science 1594-4077 1410883 http://zaguan.unizar.es/record/168599/files/texto_completo.pdf Versión publicada 1065623 http://zaguan.unizar.es/record/168599/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:168599 articulos driver 2026-02-11-10:28:00 ARTICLE