000168658 001__ 168658
000168658 005__ 20260212205631.0
000168658 0247_ $$2doi$$a10.1021/acs.est.5c14410
000168658 0248_ $$2sideral$$a148067
000168658 037__ $$aART-2025-148067
000168658 041__ $$aeng
000168658 100__ $$aAo, Tianjie
000168658 245__ $$aValorizing Every Carbon Atom: A Cascade Bioprocess for Advanced Biofuels from Corn-Stover-Derived Lignocellulose
000168658 260__ $$c2025
000168658 5203_ $$aConventional biorefining of lignocellulosic biomass, such as corn stover (CS), is hampered by poor carbon efficiency, as nearly half of the substrate carbon is lost as CO2 during ethanol fermentation. This study presents a holistically integrated cascade process designed to capture and valorize all major carbon streams. The system synergistically couples three stages: (1) high-productivity ethanol fermentation (1.68 g/L/h) using the engineered yeast Saccharomyces cerevisiae CE10; (2) anaerobic digestion of the resulting stillage, which efficiently converted residual organics into methane (171 L/kg COD) with ca. 80% COD removal; and (3) cultivation of the cyanobacterium Desertifilum tharense BERC03 using the nutrient-rich digestate and captured fermentation CO2. This integrated approach boosted the carbon utilization from a baseline of 48% to 62%. A comprehensive techno-economic analysis of an industrial-scale (2000 t/d) facility projected a Minimum Ethanol Selling Price (MESP) of $2.44 per gallon, a value approaching current market competitiveness. The analysis identified the feedstock (30%) and cellulase (17%) as the primary cost drivers. These findings demonstrate a validated biorefinery model that significantly enhances carbon recovery and outlines a viable pathway for the coproduction of multiple biofuels from lignocellulosic resources.
000168658 536__ $$9info:eu-repo/grantAgreement/ES/AEI/RYC2021-033368-I$$9info:eu-repo/grantAgreement/ES/DGA/T22-23R$$9info:eu-repo/grantAgreement/ES/MICIU/PID2023-149750OA-I00
000168658 540__ $$9info:eu-repo/semantics/closedAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000168658 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000168658 700__ $$aLuo, Yiping
000168658 700__ $$0(orcid)0000-0003-3315-5933$$aRemón, Javier$$uUniversidad de Zaragoza
000168658 700__ $$aWu, Jie
000168658 700__ $$aDeng, Fang
000168658 700__ $$aLi, Dong
000168658 700__ $$aLiu, Chenguang
000168658 700__ $$aBai, Fengwu
000168658 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000168658 773__ $$g60, 1 (2025), 566-580$$pEnviron. sci. technol.$$tENVIRONMENTAL SCIENCE & TECHNOLOGY$$x0013-936X
000168658 8564_ $$s7577634$$uhttps://zaguan.unizar.es/record/168658/files/texto_completo.pdf$$yVersión publicada$$zinfo:eu-repo/date/embargoEnd/2026-12-10
000168658 8564_ $$s3071051$$uhttps://zaguan.unizar.es/record/168658/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada$$zinfo:eu-repo/date/embargoEnd/2026-12-10
000168658 909CO $$ooai:zaguan.unizar.es:168658$$particulos$$pdriver
000168658 951__ $$a2026-02-12-20:38:55
000168658 980__ $$aARTICLE