169099 20260219091139.0 doi 10.1002/adfm.202529076 sideral 148181 ART-2026-148181 eng Del Sol-Fernández, Susel MagPiezo: A Magnetogenetic Platform for Remote Activation of Endogenous Piezo1 Channels in Endothelial Cells 2026 This study introduces MagPiezo, a novel magnetogenetic platform designed to enable remote and targeted manipulation of endogenous Piezo1 channels without genetic modification in vitro. MagPiezo integrates a custom-built magnetic applicator and 19 nm octahedral mixed ferrite nanoparticles (MF) with enhanced magnetocrystalline anisotropy, engineered to selectively target Piezo1 channels. The application of a low intensity (< 40 mT), low frequency (0.5 Hz) magnetic field is sufficient to generate wireless torque forces in the piconewton range capable of gating Piezo1 channels in vitro. Remarkably, MagPiezo induces robust responses in endothelial cells, including calcium influx, cytoskeletal reorganization and transcriptional activation. Unlike other magnetogenetic tools, MagPiezo avoids the need to assemble the magnetic nanoparticles (MNPs) into bigger beads to reach the opening threshold and does not need to genetically engineer Piezo1 channels. Moreover, it uses low magnetic field intensities and frequencies, enhancing its potential for in vivo translation. Therefore, MagPiezo is a powerful platform for manipulating Piezo1 channels and dissecting mechanotransduction events in vascular biology, paving the way for new strategies to modulate mechanoreceptors. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/AEI/CEX2023-001286-S info:eu-repo/grantAgreement/ES/DGA/E15-23R info:eu-repo/grantAgreement/EC/EU/HORIZON/MSCA-2021-PF-01-01 info:eu-repo/grantAgreement/EC/HORIZON EUROPE/101064735/EU/Tunning the force for remote magnetomechanical gating of Piezo1 channels/MAGPIEZ info:eu-repo/grantAgreement/EC/HORIZON EUROPE/101081713/EU/Intelligent Device and Computational Software to Control Mechanical Stress and Deformation for Biological Testing/ISBIOMECH info:eu-repo/grantAgreement/EC/H2020/101034263/EU/ COMFUTURO, a public-private collaboration committed to science and the future/COMFUTURO This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 101034263-COMFUTURO info:eu-repo/grantAgreement/EC/H2020/853468/EU/Remote control of cellular signalling triggered by magnetic switching/SIROCCO This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 853468-SIROCCO info:eu-repo/grantAgreement/ES/MICINN/PID2021-122508NB-I00 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion De Simone, Mariarosaria Fernández-Afonso, Yilian (orcid)0000-0002-0970-1917 Garcia-Gonzalez, Daniel Martínez-Vicente, Pablo Universidad de Zaragoza van Zanten, Thomas (orcid)0000-0002-8810-7149 Fratila, Raluca M. (orcid)0000-0001-5559-8757 Moros, Maria (orcid)0000-0002-2861-2469 1003 027 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Anatom.Embriol.Humana (2026), e29076 [20 pp.] Adv. funct. mater. Advanced Functional Materials 1616-301X 4180677 http://zaguan.unizar.es/record/169099/files/texto_completo.pdf Versión publicada 2338644 http://zaguan.unizar.es/record/169099/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:169099 articulos driver 2026-02-19-08:43:12 ARTICLE