000169155 001__ 169155
000169155 005__ 20260220162133.0
000169155 0247_ $$2doi$$a10.1016/j.actbio.2025.12.051
000169155 0248_ $$2sideral$$a148238
000169155 037__ $$aART-2026-148238
000169155 041__ $$aeng
000169155 100__ $$0(orcid)0000-0001-8946-4829$$aLaita, Nicolás
000169155 245__ $$aMechanical characterization of infarcted porcine hearts using left anterior descending and left circumflex coronary artery models
000169155 260__ $$c2026
000169155 5060_ $$aAccess copy available to the general public$$fUnrestricted
000169155 5203_ $$aThis study comprehensively analyzes passive mechanical and structural changes of cardiac tissue 6 weeks after myocardial infarction (MI) by biaxial, simple triaxial shear, and confined compression characterization. We considered a porcine model, comparing two MI types: Left Anterior Descending (LAD) artery and Left Circumflex (LCX) artery occlusions. LAD model generated apical transmurally infarcted hearts, while the LCX model generated medial heterogeneous infarctions ranging from mild locally infarcted tissue to transmurally infarcted tissue. Infarcted tissue exhibited significantly higher stiffness than healthy tissue. When peak equibiaxial stretch increases up to 20%, the stress increase is 2.19 for medial locally infarcted tissue, 4.49 for medial fully infarcted tissue, and 6.26 for apical fully infarcted tissue. Fully infarcted animals showed significant macroscopic geometrical remodeling, with thickness reductions in the infarcted area of 45%–60%, while locally infarcted tissue showed no thinning. The anisotropy of healthy myocardium was also altered post-MI: medial infarcts exhibited preferentially circumferential anisotropy, while apical infarctions increased isotropy. Histological analysis validated these mechanical alterations, revealing a substantial increase in collagen content in infarcted regions. In LCX infarctions, collagen distribution was uniformly aligned, whereas in LAD ones, its distribution was spatially heterogeneous. Non-infarcted tissue far from the infarction did not exhibit changes in mechanical properties or collagen content, suggesting a localized effect at least 6 weeks post-MI. These findings highlight the importance of considering the extent and location of MI when developing personalized therapeutic strategies.
Statement of Significance
This work provides a comprehensive mechanical and histological assessment of post-infarct porcine myocardium using advanced multimodal testing. Unlike previous studies, we compare two infarct types (LAD and LCX) under identical conditions and examine regional and severity-dependent differences in stiffness, compressibility, and anisotropy. We show that infarction induces local rather than global changes in tissue passive mechanics, modulated by infarct geometry and scar distribution. These insights support more accurate cardiac modeling and may guide personalized therapeutic strategies after myocardial infarction.
000169155 536__ $$9info:eu-repo/grantAgreement/ES/DGA/T24-20R$$9info:eu-repo/grantAgreement/EC/H2020/874827/EU/Computational biomechanics and bioengineering 3D printing to develop a personalized regenerative biological ventricular assist device to provide lasting functional support to damaged hearts/BRAV3$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 874827-BRAV3$$9info:eu-repo/grantAgreement/ES/MCIN/PLEC2021-008127$$9info:eu-repo/grantAgreement/ES/MCIN/VOLVAD_PID2022-142562OB-I00$$9info:eu-repo/grantAgreement/ES/MINECO/PID2022-140219OB-I00
000169155 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000169155 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000169155 700__ $$aAparici-Gil, Alejandro$$uUniversidad de Zaragoza
000169155 700__ $$0(orcid)0000-0001-5348-924X$$aOliván-Viguera, Aida
000169155 700__ $$aPérez-Martínez, Alba$$uUniversidad de Zaragoza
000169155 700__ $$aWu, Ming
000169155 700__ $$aGarcía de Yébenes, Manuel
000169155 700__ $$aAbizanda, Gloria
000169155 700__ $$aJanssens, Stephan
000169155 700__ $$aPrósper, Felipe
000169155 700__ $$aMazo Vega, Manuel M.
000169155 700__ $$0(orcid)0000-0002-8375-0354$$aMartínez, Miguel Ángel$$uUniversidad de Zaragoza
000169155 700__ $$0(orcid)0000-0001-8741-6452$$aDoblaré, Manuel$$uUniversidad de Zaragoza
000169155 700__ $$0(orcid)0000-0002-0664-5024$$aPeña, Estefanía$$uUniversidad de Zaragoza
000169155 7102_ $$15008$$2800$$aUniversidad de Zaragoza$$bDpto. Ingeniería Electrón.Com.$$cÁrea Teoría Señal y Comunicac.
000169155 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000169155 773__ $$g(2026), [22 pp.]$$pActa Biomater.$$tACTA BIOMATERIALIA$$x1742-7061
000169155 8564_ $$s11022867$$uhttps://zaguan.unizar.es/record/169155/files/texto_completo.pdf$$yVersión publicada
000169155 8564_ $$s2454752$$uhttps://zaguan.unizar.es/record/169155/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000169155 909CO $$ooai:zaguan.unizar.es:169155$$particulos$$pdriver
000169155 951__ $$a2026-02-20-14:54:04
000169155 980__ $$aARTICLE