169200 20260223164759.0 doi 10.1016/j.isci.2026.114751 sideral 148289 ART-2026-148289 eng Hermoso-Durán, Sonia Integrating thermal liquid biopsy, clinical data, and mass spectrometry for early diagnosis and biomarker discovery in colorectal cancer 2026 Early detection of colorectal cancer is essential to improving survival, where yet current diagnostic tools show limited performance. This study aimed to enhance diagnostic accuracy by integrating clinical variables with thermogram profiles obtained through serum-based thermal liquid biopsy and analyzed using machine learning models. We evaluated 328 patients with colorectal cancer and 355 symptomatic individuals with non-organ-specific cancer signs but negative diagnostic evaluations, to reproduce clinically relevant decision settings. The combined model showed improved classification performance compared with the use of clinical variables alone, particularly in patients with early-stage disease. In addition, proteomic analysis of samples stratified by thermogram patterns identified proteins associated with survival, including fibrinogen-like protein 1, supporting the biological relevance of these thermodynamic profiles. Together, these findings indicate that integrating serum thermogram information with routine clinical data can modestly strengthen diagnostic assessment and help identify biologically meaningful patient subgroups, offering a promising non-invasive colorectal Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/DGA/B08-24R info:eu-repo/grantAgreement/ES/DGA/B25-23R info:eu-repo/grantAgreement/ES/ISCIII-FIS/FI19-00146 info:eu-repo/grantAgreement/ES/ISCIII/PI20-00661 info:eu-repo/grantAgreement/ES/ISCIII/PI21-00394 info:eu-repo/grantAgreement/ES/MICINN/AEI/PID2021-127296OB-I00 info:eu-repo/grantAgreement/ES/MICIU/JDC2023-052992-I info:eu-repo/semantics/openAccess by-nc https://creativecommons.org/licenses/by-nc/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Ortega-Alarcón, David (orcid)0000-0003-1885-4365 Johansen, Astrid Z. McKay, Mattew J. Johansen, Julia S. Vega, Sonia (orcid)0000-0002-1232-6310 Feltoft, Claus L. Dolin, Troels Gammeltoft Lykke, Jakob Fraunhoffer, Nicolas Sánchez-Gracia, Oscar Garrido, Pablo F. Lanas, Ángel Universidad de Zaragoza (orcid)0000-0001-5932-2889 Molloy, Mark P. Velázquez-Campoy, Adrian Universidad de Zaragoza (orcid)0000-0001-5702-4538 Abian, Olga Universidad de Zaragoza (orcid)0000-0001-5664-1729 1007 610 Universidad de Zaragoza Dpto. Medicina, Psiqu. y Derm. Area Medicina 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 29, 2 (2026), 114751 [17 pp.] iScience ISCIENCE 2589-0042 4229999 http://zaguan.unizar.es/record/169200/files/texto_completo.pdf Versión publicada 1205049 http://zaguan.unizar.es/record/169200/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:169200 articulos driver 2026-02-23-14:54:23 ARTICLE