000169466 001__ 169466
000169466 005__ 20260227133541.0
000169466 0247_ $$2doi$$a10.1021/cbmi.5c00237
000169466 0248_ $$2sideral$$a148349
000169466 037__ $$aART-2026-148349
000169466 041__ $$aeng
000169466 100__ $$0(orcid)0000-0002-1791-0188$$aBonet-Aleta, Javier
000169466 245__ $$aAn Activity-Based Sensing Approach to Monitor Nanomaterial-Promoted Changes in Labile Metal Pools in Living Systems
000169466 260__ $$c2026
000169466 5060_ $$aAccess copy available to the general public$$fUnrestricted
000169466 5203_ $$aMetal-based nanoparticles are a promising class of materials for diagnosis and treatment of cancer and other diseases. However, mechanisms of action of these nanomedicines remain insufficiently understood due in large part to our limited understanding of the dynamic equilibria between solid metal nanoparticles and labile metal ions generated from these nanoparticles within complex biological milieus. Here, we apply activity-based sensing to directly identify and investigate the fate of labile copper pools with metal and oxidation state-specificity generated by anticancer copper nanomedicines. We found that treatment of cells with copper-releasing nanoparticles alter labile Cu(I)/Cu(II) ratios through an increase in labile Cu(II), while overall labile copper levels decrease. Labile copper release triggers compensatory responses in two major antioxidant pathways, glutathione (GSH) and nuclear factor erythroid 2-related factor 2 (NRF2), as well as in metal homeostasis to limit copper availability via regulation of copper export (ATP7B) and copper import (CTR1) proteins. These findings establish the value of activity-based sensing as a generalizable approach for labile metal imaging to help decipher molecular mechanisms of bioactive metal nanoparticles and guide the development of more effective nanomedicine diagnostics and therapies to target metal-dependent disease vulnerabilities.
000169466 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000169466 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000169466 700__ $$aPezacki, Aidan T.
000169466 700__ $$aOi, Miku
000169466 700__ $$0(orcid)0000-0002-4546-4111$$aHueso, Jose L.$$uUniversidad de Zaragoza
000169466 700__ $$0(orcid)0000-0002-8701-9745$$aSantamaria, Jesus$$uUniversidad de Zaragoza
000169466 700__ $$aChang, Christopher J.
000169466 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000169466 7102_ $$15005$$2790$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Tecnologi. Medio Ambiente
000169466 773__ $$g(2026), [15 pp.]$$tChemical & biomedical imaging$$x2832-3637
000169466 8564_ $$s8751743$$uhttps://zaguan.unizar.es/record/169466/files/texto_completo.pdf$$yVersión publicada
000169466 8564_ $$s3073972$$uhttps://zaguan.unizar.es/record/169466/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000169466 909CO $$ooai:zaguan.unizar.es:169466$$particulos$$pdriver
000169466 951__ $$a2026-02-27-12:36:14
000169466 980__ $$aARTICLE