000169478 001__ 169478 000169478 005__ 20260301183154.0 000169478 0247_ $$2doi$$a10.1016/j.clnu.2025.106558 000169478 0248_ $$2sideral$$a148379 000169478 037__ $$aART-2025-148379 000169478 041__ $$aeng 000169478 100__ $$aVázquez-Bolea, Natalia 000169478 245__ $$aGut microbiota composition and derived enterotypes are associated with ponderal status in preschool children. Childhood obesity risk assessment longitudinal study (CORALS) cohort 000169478 260__ $$c2025 000169478 5060_ $$aAccess copy available to the general public$$fUnrestricted 000169478 5203_ $$aSummary Background and aims Childhood obesity is a growing public health concern increasingly linked to gut microbiota. We analysed associations between microbiota composition, functionality, and weight status in 1134 children aged 3–6 years from the CORALS cohort. Methods The baseline cross-sectional study stratified participants by weight status (underweight, normal weight, overweight, obesity) and performed shotgun metagenomic sequencing of stool samples. Analyses in R assessed alpha/beta diversity, taxonomic composition, enterotypes, and microbial pathways. Results Alpha diversity decreased with increasing BMI, particularly in obesity (Shannon adj.P = 0.00301; Simpson adj.P = 0.00158). Beta diversity revealed distinct microbial structures across groups (p = 0.001). Four enterotypes were identified: obesity was associated with Enterotype 3 (Segatella-dominated, p = 0.023), while Enterotype 1 (Alistipes, Akkermansia, Coprococcus) was enriched in underweight/normal weight. Species linked to obesity included higher Phocaeicola dorei (adj.P = 0.003) and Segatella hominis (adj.P = 0.001), and lower Longicatena caecimuris (adj.P = 0.03) and Blautia parvula (adj.P = 0.003). Functional analyses showed downregulation of vitamin and nucleotide biosynthesis pathways and reduced carbohydrate metabolism in overweight/obesity. Conclusions Gut microbiota composition and functionality are strongly associated with weight status in early childhood, suggesting microbial biomarkers and metabolic pathways relevant to understand early obesity development. 000169478 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/CEX2021-001189-S$$9info:eu-repo/grantAgreement/EUR/MICINN/PID2023-150693OB 000169478 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 000169478 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000169478 700__ $$aMora-Martínez, Carlos 000169478 700__ $$aCuervo, Marta 000169478 700__ $$aMartinez, J. Alfredo 000169478 700__ $$aGil-Campos, Mercedes 000169478 700__ $$aLeis, Rosaura 000169478 700__ $$aBabio, Nancy 000169478 700__ $$aMoreno, Luis A. 000169478 700__ $$aCorella, Dolores 000169478 700__ $$aMoreira Echeverria, Ana 000169478 700__ $$aAguilera, Concepcion M. 000169478 700__ $$aCastro-Collado, Cristina 000169478 700__ $$aPicáns-Leis, Rosaura 000169478 700__ $$aHernández-Cacho, Adrián 000169478 700__ $$aMiguel-Berges, Maria L. 000169478 700__ $$aMartin-Climent, Paula 000169478 700__ $$aJurado-Castro, Jose Manuel 000169478 700__ $$aVázquez-Cobela, Rocío 000169478 700__ $$aPlaza-Diaz, Julio 000169478 700__ $$aRueda-De Torre, Isabel$$uUniversidad de Zaragoza 000169478 700__ $$aPastor-Villaescusa, Belén 000169478 700__ $$ade la Torre-Aguilar, Maria José 000169478 700__ $$aSalas-Salvadó, Jordi 000169478 700__ $$aSanz, Yolanda 000169478 700__ $$aNavas-Carretero, Santiago 000169478 7102_ $$11011$$2615$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Medic.Prevent.Salud Públ. 000169478 773__ $$g57 (2025), 106558 [14 pp.]$$pClin. nutr.$$tClinical Nutrition$$x0261-5614 000169478 8564_ $$s5959917$$uhttps://zaguan.unizar.es/record/169478/files/texto_completo.pdf$$yVersión publicada 000169478 8564_ $$s2605509$$uhttps://zaguan.unizar.es/record/169478/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000169478 909CO $$ooai:zaguan.unizar.es:169478$$particulos$$pdriver 000169478 951__ $$a2026-03-01-17:54:17 000169478 980__ $$aARTICLE