169478 20260301183154.0 doi 10.1016/j.clnu.2025.106558 sideral 148379 ART-2025-148379 eng Vázquez-Bolea, Natalia Gut microbiota composition and derived enterotypes are associated with ponderal status in preschool children. Childhood obesity risk assessment longitudinal study (CORALS) cohort 2025 Summary Background and aims Childhood obesity is a growing public health concern increasingly linked to gut microbiota. We analysed associations between microbiota composition, functionality, and weight status in 1134 children aged 3–6 years from the CORALS cohort. Methods The baseline cross-sectional study stratified participants by weight status (underweight, normal weight, overweight, obesity) and performed shotgun metagenomic sequencing of stool samples. Analyses in R assessed alpha/beta diversity, taxonomic composition, enterotypes, and microbial pathways. Results Alpha diversity decreased with increasing BMI, particularly in obesity (Shannon adj.P = 0.00301; Simpson adj.P = 0.00158). Beta diversity revealed distinct microbial structures across groups (p = 0.001). Four enterotypes were identified: obesity was associated with Enterotype 3 (Segatella-dominated, p = 0.023), while Enterotype 1 (Alistipes, Akkermansia, Coprococcus) was enriched in underweight/normal weight. Species linked to obesity included higher Phocaeicola dorei (adj.P = 0.003) and Segatella hominis (adj.P = 0.001), and lower Longicatena caecimuris (adj.P = 0.03) and Blautia parvula (adj.P = 0.003). Functional analyses showed downregulation of vitamin and nucleotide biosynthesis pathways and reduced carbohydrate metabolism in overweight/obesity. Conclusions Gut microbiota composition and functionality are strongly associated with weight status in early childhood, suggesting microbial biomarkers and metabolic pathways relevant to understand early obesity development. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/MICINN/CEX2021-001189-S info:eu-repo/grantAgreement/EUR/MICINN/PID2023-150693OB info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Mora-Martínez, Carlos Cuervo, Marta Martinez, J. Alfredo Gil-Campos, Mercedes Leis, Rosaura Babio, Nancy Moreno, Luis A. Corella, Dolores Moreira Echeverria, Ana Aguilera, Concepcion M. Castro-Collado, Cristina Picáns-Leis, Rosaura Hernández-Cacho, Adrián Miguel-Berges, Maria L. Martin-Climent, Paula Jurado-Castro, Jose Manuel Vázquez-Cobela, Rocío Plaza-Diaz, Julio Rueda-De Torre, Isabel Universidad de Zaragoza Pastor-Villaescusa, Belén de la Torre-Aguilar, Maria José Salas-Salvadó, Jordi Sanz, Yolanda Navas-Carretero, Santiago 1011 615 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Medic.Prevent.Salud Públ. 57 (2025), 106558 [14 pp.] Clin. nutr. Clinical Nutrition 0261-5614 5959917 http://zaguan.unizar.es/record/169478/files/texto_completo.pdf Versión publicada 2605509 http://zaguan.unizar.es/record/169478/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:169478 articulos driver 2026-03-01-17:54:17 ARTICLE