000169495 001__ 169495
000169495 005__ 20260302165029.0
000169495 0247_ $$2doi$$a10.1007/s11259-026-11117-3
000169495 0248_ $$2sideral$$a148421
000169495 037__ $$aART-2026-148421
000169495 041__ $$aeng
000169495 100__ $$0(orcid)0000-0001-6209-4282$$aVillanueva-Saz, Sergio$$uUniversidad de Zaragoza
000169495 245__ $$aEvaluation of a commercial multispecies rapid test for anti-Leishmania infantum antibodies in unconventional animal species
000169495 260__ $$c2026
000169495 5060_ $$aAccess copy available to the general public$$fUnrestricted
000169495 5203_ $$aLeishmania infantum is a vector-borne protozoan causing zoonotic visceral leishmaniasis in the Mediterranean basin, where dogs are the primary reservoir. However, infection in various domestic and wild mammals raises questions about their epidemiological roles. Reliable diagnosis in non-canine species remains challenging, as most serological assays are developed for dogs and cats. This study evaluated a commercially available multispecies rapid immunochromatographic test (Uranotest® Leishmania feline) for detecting anti-Leishmania antibodies in a diverse panel of domestic and non-domestic animals. A total of 186 serum samples from different species were analyzed, previously classified as seropositive (n = 65) or seronegative (n = 121) using an in-house ELISA as reference. Immunochromatographic rapid test performance was compared to ELISA results using Cohen’s kappa to assess agreement. Overall agreement reached 82.3% (κ = 0.56), indicating moderate concordance. This immunochromatographic test correctly identified all positive and negative canine and feline samples and showed perfect agreement in other carnivores such as mink and wolves. In contrast, all herbivorous species, including alpaca, horses, goats, llama, sheep, bison, and dromedaries, produced negative results by the rapid test despite ELISA seropositivity, suggesting limited affinity of the conjugate for herbivore immunoglobulins. These findings indicate that, while the assayed rapid test is suitable for detecting anti-Leishmania antibodies in non-herbivorous species, it is unreliable for herbivores. The study highlights the importance of species-specific validation of serological assays and suggests that rapid tests using protein A conjugates may have restricted cross-species utility, reinforcing the need for adapted diagnostic tools in multispecies and wildlife contexts.
000169495 536__ $$9info:eu-repo/grantAgreement/ES/DGA/AR15-32R
000169495 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000169495 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000169495 700__ $$0(orcid)0000-0002-8727-0323$$aGonzález, Ana$$uUniversidad de Zaragoza
000169495 700__ $$0(orcid)0000-0003-2555-8425$$aPérez, María D.$$uUniversidad de Zaragoza
000169495 700__ $$0(orcid)0000-0002-7822-6646$$aLacasta, Delia$$uUniversidad de Zaragoza
000169495 700__ $$0(orcid)0000-0002-2557-4890$$aFernández, Antonio$$uUniversidad de Zaragoza
000169495 700__ $$0(orcid)0009-0003-9757-3840$$aQuilez, Pablo$$uUniversidad de Zaragoza
000169495 700__ $$0(orcid)0000-0002-0313-5926$$aOrtín, Aurora$$uUniversidad de Zaragoza
000169495 700__ $$aGuallar, David$$uUniversidad de Zaragoza
000169495 700__ $$0(orcid)0000-0002-9723-9004$$aGómez, Álex$$uUniversidad de Zaragoza
000169495 700__ $$aMarteles-Aragüés, Diana$$uUniversidad de Zaragoza
000169495 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000169495 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000169495 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000169495 773__ $$g50, 181 (2026), 7$$pVet. res. commun.$$tVeterinary Research Communications$$x0165-7380
000169495 8564_ $$s828975$$uhttps://zaguan.unizar.es/record/169495/files/texto_completo.pdf$$yVersión publicada
000169495 8564_ $$s2424886$$uhttps://zaguan.unizar.es/record/169495/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000169495 909CO $$ooai:zaguan.unizar.es:169495$$particulos$$pdriver
000169495 951__ $$a2026-03-02-14:47:50
000169495 980__ $$aARTICLE