000169906 001__ 169906
000169906 005__ 20260306154908.0
000169906 0247_ $$2doi$$a10.1038/s41467-026-68786-6
000169906 0248_ $$2sideral$$a148443
000169906 037__ $$aART-2026-148443
000169906 041__ $$aeng
000169906 100__ $$0(orcid)0000-0001-9224-5854$$aTaleb, Víctor
000169906 245__ $$aPlant fucosyltransferase FUT11 distorts the sugar acceptor to catalyze via a transient oxocarbenium intermediate mechanism
000169906 260__ $$c2026
000169906 5060_ $$aAccess copy available to the general public$$fUnrestricted
000169906 5203_ $$aGlycosyltransferases catalyze glycosidic bond formation by activating the donor sugar, while the sugar acceptor substrate is considered passive, maintaining a chair conformation during catalysis. We challenge this through a multidisciplinary study of Arabidopsis thaliana FUT11, a core α1,3-fucosyltransferase essential for plant development and reproduction. AtFUT11 adopts a GT-B fold with an additional N-terminal subdomain that anchors the G0 N-glycan, while the α1,3 arm is mainly recognized by the acceptor Rossmann subdomain. The α1,6 arm remains solvent-exposed, allowing diverse modifications, while solvent exposure of the central mannose’s OH2 explains tolerance for β1,2-xylose. Remarkably, simulations suggest the catalytic base Glu158 may promote the innermost GlcNAc’s transient puckering distortion to align the hydroxyl for nucleophilic attack. This enables an asynchronous SN2-like mechanism bordering SN1 character, with formation of a transient oxocarbenium ion triggered by pyrophosphate departure, followed by nucleophilic attack coupled with proton transfer. Homology with human FUT9 explains AtFUT11’s side activity on LacNAc, revealing plasticity and evolutionary convergence between plant and mammalian antenna-fucosyltransferases.
000169906 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FEDER/E34-R17$$9info:eu-repo/grantAgreement/ES/DGA/LMP58_18$$9info:eu-repo/grantAgreement/ES/MICINN AEI/PID2022-136362NB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2022-137973NB-I00
000169906 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000169906 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000169906 700__ $$aSanz-Martínez, Ignacio
000169906 700__ $$aSerna, Sonia
000169906 700__ $$aBort-Griñó, María
000169906 700__ $$aNarimatsu, Yoshiki
000169906 700__ $$aFurukawa, Sanae
000169906 700__ $$aReichardt, Niels C.
000169906 700__ $$aClausen, Henrik
000169906 700__ $$0(orcid)0000-0002-2202-3460$$aMerino, Pedro$$uUniversidad de Zaragoza
000169906 700__ $$0(orcid)0000-0002-3122-9401$$aHurtado-Guerrero, Ramon
000169906 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000169906 773__ $$g17 (2026), 1960 [18 pp.]$$tNature communications$$x2041-1723
000169906 8564_ $$s3351047$$uhttps://zaguan.unizar.es/record/169906/files/texto_completo.pdf$$yVersión publicada
000169906 8564_ $$s2542500$$uhttps://zaguan.unizar.es/record/169906/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000169906 909CO $$ooai:zaguan.unizar.es:169906$$particulos$$pdriver
000169906 951__ $$a2026-03-06-14:50:13
000169906 980__ $$aARTICLE