170071 20260316092630.0 doi 10.1021/acsbiomaterials.5c01488 sideral 148607 ART-2026-148607 eng Kulczynska, Julia Biogenesis of Au Nanoparticles from Plant-Derived Metabolites – In Vitro and In Vivo StudiesClick to copy article link 2026 Biomimetic gold nanoparticles (Au NPs) were synthesized via a sustainable approach without any additional toxic chemical reagents and fully characterized. It was proven that only whole aqueous extracts of Rosa damascene (RD) and Rosa rugosa (RR) are powerful enough to reduce, graft, and stabilize metallic nanostructures, resulting in the formation of stable, monodisperse nanocolloids (Au@RD NPs and Au@RR NPs) whereas individual constituent molecules were insufficient to yield stable metal NPs. The biological study conducted, both in vitro and in vivo, revealed no acute cytotoxicity (in HaCaT cell lines and zebrafish larval models) but bacteriostatic activity at equivalent doses with potent inhibition of biofilm formation (for a MRSA strain). Noteworthy, the additive antibacterial activity of rose extracts when combined with rifampicin promotes that these attractive inorganic–organic hybrids could be suitable alternatives to combat the acquisition of antimicrobial resistance. This huge application potential was also emphasized by the presence of insignificant changes in the expression of pro-inflammatory cytokine genes (IL-1β, IL-6, and CXCL8) and apoptotic/autophagic associated genes (TP53, MAP1LC3B, and SQSTM1) in treated HaCaT cells at antimicrobial doses. In addition, at the studied doses, the survival of Danio rerio larvae and their proper development (i.e., lack of deformities) endorsed biocompatibility in vivo. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/AEI/CEX2023-001286-S info:eu-repo/grantAgreement/ES/MICINN/PID2023-146091OB-I00 info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Topa, Natalia Widziolek, Magdalena Homa, Joanna Kwiecien, Inga Gamez, Enrique (orcid)0000-0002-0272-3152 Arruebo, Manuel Universidad de Zaragoza (orcid)0000-0003-3165-0156 Kyziol, Agnieszka 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 12, 3 (2026), 1508-1521 ACS biomater. sci. eng. ACS BIOMATERIALS SCIENCE & ENGINEERING 2373-9878 9228922 http://zaguan.unizar.es/record/170071/files/texto_completo.pdf Versión publicada 3389248 http://zaguan.unizar.es/record/170071/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:170071 articulos driver 2026-03-16-08:18:04 ARTICLE