000170086 001__ 170086
000170086 005__ 20260318155254.0
000170086 0247_ $$2doi$$a10.1021/acsanm.5c05265
000170086 0248_ $$2sideral$$a148649
000170086 037__ $$aART-2026-148649
000170086 041__ $$aeng
000170086 100__ $$aAlonso-Campos, Pablo$$uUniversidad de Zaragoza
000170086 245__ $$aMicrofluidic-Assisted Coating of Nanoparticles by Membranes from Extracellular Vesicles
000170086 260__ $$c2026
000170086 5060_ $$aAccess copy available to the general public$$fUnrestricted
000170086 5203_ $$aExtracellular vesicle (EV)-based nanotherapies represent a promising alternative in cancer nanomedicine thanks to their targeting properties. However, two major issues remain when combining nanoparticles (NPs) with EVs: (1) safety concerns of cancer-derived EVs related to their oncogenic nature and (2) lack of robust, reproducible, and efficient strategies for NPs coating with EVs membranes. To overcome these main challenges, in this study, we have combined a microfluidic platform to direct the reassembly of previously isolated EVs-derived membranes from lung cancer cells onto fluorescent silica NPs (FSNs). These FSNs leverage the versatile surface functionalization of silica NPs together with intrinsic fluorescent properties for precise intracellular tracking of our hybrids, avoiding membrane-labeling fluorescent probes. While preserving the tumor-targeting properties of natural lung cancer-derived EVs, the proposed microfluidic strategy offers superior handling and manipulation of the process to guarantee (1) homogeneous EV-like coating (6.3 nm thick), (2) high reproducibility, (3) efficient coating yields (87.8% of the NPs were coated), and (4) selective tumor-targeting properties (8.0- and 4.3-fold increases for parental A549 tumoral cells compared to HeLa and fibroblasts at 4 h, respectively). This technology enables the fabrication of biomimetic hybrid core−shell structures with high precision and stability in a continuous process that is more amenable to scaling up.
000170086 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-127847OB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2024–160339OB-I00$$9info:eu-repo/grantAgreement/ES/MICIU/RYC2024-050017-I
000170086 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000170086 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000170086 700__ $$0(orcid)0000-0001-5512-0075$$aBalas, Francisco$$uUniversidad de Zaragoza
000170086 700__ $$0(orcid)0000-0002-4546-4111$$aHueso, Jose L.$$uUniversidad de Zaragoza
000170086 700__ $$0(orcid)0000-0002-6873-5244$$aSebastián, Víctor$$uUniversidad de Zaragoza
000170086 700__ $$0(orcid)0000-0001-8762-5457$$aSancho-Albero, María$$uUniversidad de Zaragoza
000170086 700__ $$0(orcid)0000-0002-8701-9745$$aSantamaría, Jesus$$uUniversidad de Zaragoza
000170086 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000170086 7102_ $$15005$$2790$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Tecnologi. Medio Ambiente
000170086 773__ $$g(2026), [15 pp.]$$pACS appl. nano mater.$$tACS APPLIED NANO MATERIALS$$x2574-0970
000170086 8564_ $$s13106661$$uhttps://zaguan.unizar.es/record/170086/files/texto_completo.pdf$$yVersión publicada
000170086 8564_ $$s3151675$$uhttps://zaguan.unizar.es/record/170086/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000170086 909CO $$ooai:zaguan.unizar.es:170086$$particulos$$pdriver
000170086 951__ $$a2026-03-18-13:51:55
000170086 980__ $$aARTICLE