170086 20260318155254.0 doi 10.1021/acsanm.5c05265 sideral 148649 ART-2026-148649 eng Alonso-Campos, Pablo Universidad de Zaragoza Microfluidic-Assisted Coating of Nanoparticles by Membranes from Extracellular Vesicles 2026 Extracellular vesicle (EV)-based nanotherapies represent a promising alternative in cancer nanomedicine thanks to their targeting properties. However, two major issues remain when combining nanoparticles (NPs) with EVs: (1) safety concerns of cancer-derived EVs related to their oncogenic nature and (2) lack of robust, reproducible, and efficient strategies for NPs coating with EVs membranes. To overcome these main challenges, in this study, we have combined a microfluidic platform to direct the reassembly of previously isolated EVs-derived membranes from lung cancer cells onto fluorescent silica NPs (FSNs). These FSNs leverage the versatile surface functionalization of silica NPs together with intrinsic fluorescent properties for precise intracellular tracking of our hybrids, avoiding membrane-labeling fluorescent probes. While preserving the tumor-targeting properties of natural lung cancer-derived EVs, the proposed microfluidic strategy offers superior handling and manipulation of the process to guarantee (1) homogeneous EV-like coating (6.3 nm thick), (2) high reproducibility, (3) efficient coating yields (87.8% of the NPs were coated), and (4) selective tumor-targeting properties (8.0- and 4.3-fold increases for parental A549 tumoral cells compared to HeLa and fibroblasts at 4 h, respectively). This technology enables the fabrication of biomimetic hybrid core−shell structures with high precision and stability in a continuous process that is more amenable to scaling up. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/MICINN/PID2021-127847OB-I00 info:eu-repo/grantAgreement/ES/MICINN/PID2024–160339OB-I00 info:eu-repo/grantAgreement/ES/MICIU/RYC2024-050017-I info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Balas, Francisco Universidad de Zaragoza (orcid)0000-0001-5512-0075 Hueso, Jose L. Universidad de Zaragoza (orcid)0000-0002-4546-4111 Sebastián, Víctor Universidad de Zaragoza (orcid)0000-0002-6873-5244 Sancho-Albero, María Universidad de Zaragoza (orcid)0000-0001-8762-5457 Santamaría, Jesus Universidad de Zaragoza (orcid)0000-0002-8701-9745 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 5005 790 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Tecnologi. Medio Ambiente (2026), [15 pp.] ACS appl. nano mater. ACS APPLIED NANO MATERIALS 2574-0970 13106661 http://zaguan.unizar.es/record/170086/files/texto_completo.pdf Versión publicada 3151675 http://zaguan.unizar.es/record/170086/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:170086 articulos driver 2026-03-18-13:51:55 ARTICLE