000170088 001__ 170088
000170088 005__ 20260318155254.0
000170088 0247_ $$2doi$$a10.1016/j.vetmic.2026.110964
000170088 0248_ $$2sideral$$a148652
000170088 037__ $$aART-2026-148652
000170088 041__ $$aeng
000170088 100__ $$aSaralegui, Luis$$uUniversidad de Zaragoza
000170088 245__ $$aEngineering bright and genetically stable fluorescent Streptococcus suis strains for functional in vitro and in vivo applications
000170088 260__ $$c2026
000170088 5060_ $$aAccess copy available to the general public$$fUnrestricted
000170088 5203_ $$aStreptococcus suis is a major cause of streptococcal infections in pigs and an emerging zoonotic pathogen, resulting in substantial economic losses in the swine production industry. The limited efficacy of current vaccine strategies and the rise in antimicrobial resistance have intensified efforts to investigate the biology and pathogenesis of the microorganism as a basis for developing alternative control strategies. In this work, we engineered a genetically stable S. suis strain producing a superfolder green fluorescent protein that may serve to study this pathogen in a variety of in vitro and in vivo assays. Multiple S. suis strains from different genetic backgrounds were successfully transformed, exhibiting strong and stable fluorescence without compromising bacterial growth. Fluorescence intensity remained consistent over 15 serial passages in culture without the need for antibiotic selection, supporting its suitability for long-term experiments. The fluorescent strains were nicely distinguishable by fluorescence microscopy and enabled the detailed study of various biological aspects, including biofilm formation, interactions with eukaryotic cells, and differential growth. In murine infection models, the engineered strains caused streptococcal disease, unlike unencapsulated mutant derivatives, and were detected in internal organs via fluorescence microscopy. Altogether, this work provides a valuable tool for advancing research into S. suis.
000170088 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/PID2023-146823OB-I00
000170088 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000170088 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000170088 700__ $$aGarcía, Carla$$uUniversidad de Zaragoza
000170088 700__ $$aJurado, Paula
000170088 700__ $$aBosch, Camila
000170088 700__ $$avan Setten, Marga
000170088 700__ $$aWessel, Vloet
000170088 700__ $$aBermúdez, Rocío
000170088 700__ $$0(orcid)0000-0002-1974-9025$$aMarín, Clara
000170088 700__ $$0(orcid)0000-0002-8134-0693$$aArenas, Jesús$$uUniversidad de Zaragoza
000170088 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000170088 773__ $$g316 (2026), 110964 [12 pp.]$$pVet. microbiol.$$tVeterinary Microbiology$$x0378-1135
000170088 8564_ $$s7199980$$uhttps://zaguan.unizar.es/record/170088/files/texto_completo.pdf$$yVersión publicada
000170088 8564_ $$s2554922$$uhttps://zaguan.unizar.es/record/170088/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000170088 909CO $$ooai:zaguan.unizar.es:170088$$particulos$$pdriver
000170088 951__ $$a2026-03-18-13:51:58
000170088 980__ $$aARTICLE