170142 20260407115446.0 doi 10.1016/j.isci.2026.115292 sideral 148677 ART-2026-148677 eng Sanz-García, Fernando Universidad de Zaragoza An assay toolkit for anti-tuberculosis drugs in the hollow fiber system 2026 The hollow-fiber system for tuberculosis (HFS-TB) is an in vitro pharmacokinetic/pharmacodynamic (PKPD) tool qualified by the European Medicines Agency to support anti-TB drug development. It can simulate PK parameters of antimicrobials and feed in silico models to inform Phase II/III clinical trials. Yet, its implementation is challenging due to lack of consolidated technical guidelines, such as the compatibility of drugs with the most commonly used types of HFS-TB cartridges. Herein, we uncovered the compatibility of 10 anti-TB drugs alone: bedaquiline, clarithromycin, delamanid, ethambutol, isoniazid, linezolid, moxifloxacin, pretomanid, rifampicin and rifapentine; and a combination: bedaquiline-pretomanid-linezolid. The most hydrophilic compounds were within the compatibility range with all fibers; whereas the lipophilic ones required adjustments to be used in the system. Also, polysulfone and cellulose fibers were the most suitable for the tested drugs. Our data strengthen the importance of these preliminary studies and provide a useful toolkit towards wider HFS-TB implementation. Access copy available to the general public Unrestricted info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ info:eu-repo/semantics/article info:eu-repo/semantics/submittedVersion Ndjogou, Dominique Aguilar-Ayala, Diana A. Universidad de Zaragoza Eveque-Mourroux, Maxime R. Benítez, Ana Universidad de Zaragoza (orcid)0009-0000-8481-7453 Aznar, Sergio Universidad de Zaragoza Isach-Traver, Nuria Mendoza-Losana, Alfonso Willand, Nicolas Lucía, Ainhoa Universidad de Zaragoza (orcid)0000-0002-0111-4697 Ramón-García, Santiago (orcid)0000-0002-8480-0325 ERA4TB Consortium 1011 630 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Microbiología 1007 610 Universidad de Zaragoza Dpto. Medicina, Psiqu. y Derm. Area Medicina 0 X Universidad de Zaragoza Grupos de investigación G.I. Genética de Micob GENMICO (2026), 115292 [51 pp.] iScience ISCIENCE 2589-0042 3542070 http://zaguan.unizar.es/record/170142/files/texto_completo.pdf Preprint 959155 http://zaguan.unizar.es/record/170142/files/texto_completo.jpg?subformat=icon icon Preprint oai:zaguan.unizar.es:170142 articulos driver 2026-03-26-14:30:22 ARTICLE