000170172 001__ 170172
000170172 005__ 20260407115448.0
000170172 0247_ $$2doi$$a10.1039/d5nh00782h
000170172 0248_ $$2sideral$$a148745
000170172 037__ $$aART-2026-148745
000170172 041__ $$aeng
000170172 100__ $$aEsteban-Pérez, Natalia
000170172 245__ $$aControlled release of glucocorticoid via PLGA nanoparticles for modulating macrophage polarization in inflammation situations
000170172 260__ $$c2026
000170172 5060_ $$aAccess copy available to the general public$$fUnrestricted
000170172 5203_ $$aGlucocorticoids are among the most widely used anti-inflammatory and immunosuppressive drugs. However, their prolonged administration is associated with a wide range of adverse side effects including long-lasting immunosuppression. In this study, we aimed to encapsulate two commonly used glucocorticoids with different potency and duration, hydrocortisone and dexamethasone, into poly(lactic-co-glycolic acid) (PLGA) nanoparticles with the goal to modulate inflammatory gene expression in a delivery-dependent manner. We evaluated their anti-inflammatory properties in two in vitro models varying the timing of treatment administration based on lipopolysaccharide M1-polarized macrophages, key effectors of the innate immune system. Our results demonstrated that, for both strategies, drug-loaded nanoparticles significantly reduced the expression of interleukin-6, a pro-inflammatory cytokine, compared to the free drugs. However, in one of the strategies, while free drugs induced upregulation of interleukin-10, a key anti-inflammatory cytokine, no such effect was observed with the nanoparticle-based formulations. Overall, these results demonstrate that PLGA nanoparticles enable sustained glucocorticoid delivery and modulate inflammatory gene expression in activated macrophages in a delivery- and timing-dependent manner, providing comparative insight into how glucocorticoid delivery via PLGA nanoparticles shapes inflammatory gene regulation depending on treatment timing and highlighting the importance of in vitro model design.
000170172 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E15-20R$$9info:eu-repo/grantAgreement/EC/HORIZON EUROPE/101064735/EU/Tunning the force for remote magnetomechanical gating of Piezo1 channels/MAGPIEZ$$9info:eu-repo/grantAgreement/ES/ISCIII/CB16-01/00263$$9info:eu-repo/grantAgreement/ES/MICINN/CEX2023-001286-S$$9info:eu-repo/grantAgreement/ES/MICINN/PID2020-118485RB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2022-141276OB-I00
000170172 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttps://creativecommons.org/licenses/by-nc/4.0/deed.es
000170172 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000170172 700__ $$aDel Sol-Fernández, Susel
000170172 700__ $$0(orcid)0000-0003-0702-8260$$aMartín-Rapún, Rafael$$uUniversidad de Zaragoza
000170172 700__ $$0(orcid)0000-0003-1081-8482$$aMartínez de la Fuente, Jesús
000170172 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000170172 773__ $$g(2026), [10 pp.]$$pNanoscale Horizons$$tNanoscale Horizons$$x2055-6756
000170172 8564_ $$s2790944$$uhttps://zaguan.unizar.es/record/170172/files/texto_completo.pdf$$yVersión publicada
000170172 8564_ $$s2868993$$uhttps://zaguan.unizar.es/record/170172/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000170172 909CO $$ooai:zaguan.unizar.es:170172$$particulos$$pdriver
000170172 951__ $$a2026-03-26-14:31:00
000170172 980__ $$aARTICLE