000170179 001__ 170179
000170179 005__ 20260407115449.0
000170179 0247_ $$2doi$$a10.1007/s11259-026-11159-7
000170179 0248_ $$2sideral$$a148731
000170179 037__ $$aART-2026-148731
000170179 041__ $$aeng
000170179 100__ $$aDidkowska, Anna
000170179 245__ $$aFirst report of serological and molecular detection of influenza a (h1n1) in european bison (bison bonasus)
000170179 260__ $$c2026
000170179 5060_ $$aAccess copy available to the general public$$fUnrestricted
000170179 5203_ $$aInfluenza A virus is a zoonotic pathogen capable of infecting a wide range of hosts, yet data on infections in wild ruminants are limited. The European bison (Bison bonasus), Europe’s largest terrestrial mammal, had not previously been identified as a host for Influenza A virus. This study aimed to provide the first molecular and serological evidence of H1N1 pandemic influenza A virus (A/H1N1pdm) infection in European bison, contributing to the understanding of Influenza A virus ecology at the wildlife–livestock interface. We retrospectively analysed 335 serum samples collected from European bison between 2017 and 2023 using a commercial ELISA detecting antibodies against all Influenza A virus subtypes. Tissue samples (heart and liver) from ELISA-positive animal were further tested by RT-qPCR targeting hemagglutinin (HA) and neuraminidase (NA) genes of A/H1N1pdm, A/H3N2, and A/H5N1. PCR-positive products were sequenced for confirmation. Seroprevalence of Influenza A virus was 0.3% (1/335; 95% CI: 0.02–1.71). Influenza A virus RNA (A/H1N1pdm) was detected in the heart and liver tissue of a 4-year-old captive male from Borecka Forest, culled in 2018. The animal exhibited pulmonary congestion, emphysema, and posthitis at necropsy. This study provides the first molecular and serological confirmation of H1N1pdm infection in European bison. The findings highlight the potential susceptibility of this species to Influenza A virus and raise questions about possible transmission routes, including spillover from domestic animals or humans.
000170179 536__ $$9info:eu-repo/grantAgreement/ES/DGA/AR15-32R
000170179 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000170179 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000170179 700__ $$aMartín-Santander, Víctor
000170179 700__ $$aGolke, Anna
000170179 700__ $$aWojciechowska, Marlena
000170179 700__ $$aOlech, Wanda
000170179 700__ $$aSzalus-Jordanow, Olga
000170179 700__ $$aDzieciatkowski, Tomasz
000170179 700__ $$0(orcid)0000-0002-9723-9004$$aGómez, Álex$$uUniversidad de Zaragoza
000170179 700__ $$0(orcid)0000-0001-6209-4282$$aVillanueva-Saz, Sergio$$uUniversidad de Zaragoza
000170179 700__ $$aKwiecien, Ewelina
000170179 700__ $$0(orcid)0009-0003-9757-3840$$aQuílez, Pablo$$uUniversidad de Zaragoza
000170179 700__ $$aAnusz, Krzysztof
000170179 700__ $$aMarteles-Aragüés, Diana$$uUniversidad de Zaragoza
000170179 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000170179 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000170179 773__ $$g50, 212 (2026), 1-6$$pVet. res. commun.$$tVeterinary Research Communications$$x0165-7380
000170179 8564_ $$s1443557$$uhttps://zaguan.unizar.es/record/170179/files/texto_completo.pdf$$yVersión publicada
000170179 8564_ $$s2026224$$uhttps://zaguan.unizar.es/record/170179/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000170179 909CO $$ooai:zaguan.unizar.es:170179$$particulos$$pdriver
000170179 951__ $$a2026-03-26-14:31:08
000170179 980__ $$aARTICLE