000170257 001__ 170257
000170257 005__ 20260410165450.0
000170257 0247_ $$2doi$$a10.1016/j.funbio.2023.07.004
000170257 0248_ $$2sideral$$a135340
000170257 037__ $$aART-2023-135340
000170257 041__ $$aeng
000170257 100__ $$aGómez-Molina, Eva
000170257 245__ $$aSequential application of inoculation methods improves mycorrhization of Quercus ilex seedlings by Tuber melanosporum
000170257 260__ $$c2023
000170257 5060_ $$aAccess copy available to the general public$$fUnrestricted
000170257 5203_ $$aThe use of mycorrhized seedlings has been critical to the success of modern truffle cultivation, which nowadays supplies most European black truffles (Tuber melanosporum) to markets. Ascospore inoculation has been traditionally used to produce these seedlings, but little scientific information is publicly available on the inoculation methods applied or on the possibility of combining them. We evaluated the potential of sequential inoculation for the controlled colonization of holm oak fine roots by T. melanosporum, with two different nursery assays and a full factorial design. Three inoculation methods were sequentially applied: radicle inoculation, inoculation of the substrate in seedling trays and inoculation of the substrate in the final pot. Despite the differences in the results of the two assays, which suggest that cultivation conditions and/or the timing of nursery operations may influence the relative effectiveness of inoculation methods, the sequential application appeared as an effective and realistic alternative for commercial inoculation of holm oak seedlings with T. melanosporum. The increase in the amount of inoculum applied with each inoculation method improved the mycorrhizal colonization of seedlings, whereas separately none of the inoculation methods appeared clearly superior to the other ones. The depth distribution of truffle mycorrhizae pointed that the inoculation in the final pot was more effective than other methods in lower parts of the root system, whereas the early inoculation appeared more effective to reduce the occurrence of the opportunist ectomycorrhizal fungus Sphaerosporella brunnea.
000170257 536__ $$9info:eu-repo/grantAgreement/EC/H2020/101007623/EU/INnovation in Truffle cultivation, preservAtion, proCessing and wild truffle resources managemenT/INTACT$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 101007623-INTACT
000170257 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000170257 590__ $$a2.9$$b2023
000170257 591__ $$aMYCOLOGY$$b12 / 33 = 0.364$$c2023$$dQ2$$eT2
000170257 592__ $$a0.696$$b2023
000170257 593__ $$aPlant Science$$c2023$$dQ1
000170257 593__ $$aEcology, Evolution, Behavior and Systematics$$c2023$$dQ2
000170257 593__ $$aInfectious Diseases$$c2023$$dQ2
000170257 593__ $$aGenetics$$c2023$$dQ3
000170257 594__ $$a5.8$$b2023
000170257 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000170257 700__ $$0(orcid)0000-0003-4331-9794$$aSánchez, Sergio$$uUniversidad de Zaragoza
000170257 700__ $$aPuig-Pey, Meritxell
000170257 700__ $$0(orcid)0000-0002-7248-234X$$aGarcía-Barreda, Sergi$$uUniversidad de Zaragoza
000170257 7102_ $$15011$$2500$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cArea Ingeniería Agroforestal
000170257 7102_ $$15011$$2705$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Producción Vegetal
000170257 773__ $$g127, 10-11 (2023), 1328-1335$$pFUNGAL BIOLOGY$$tFUNGAL BIOLOGY$$x1878-6146
000170257 8564_ $$s528102$$uhttps://zaguan.unizar.es/record/170257/files/texto_completo.pdf$$yPostprint
000170257 8564_ $$s422670$$uhttps://zaguan.unizar.es/record/170257/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000170257 909CO $$ooai:zaguan.unizar.es:170257$$particulos$$pdriver
000170257 951__ $$a2026-04-10-13:45:19
000170257 980__ $$aARTICLE