170257 20260410165450.0 doi 10.1016/j.funbio.2023.07.004 sideral 135340 ART-2023-135340 eng Gómez-Molina, Eva Sequential application of inoculation methods improves mycorrhization of Quercus ilex seedlings by Tuber melanosporum 2023 The use of mycorrhized seedlings has been critical to the success of modern truffle cultivation, which nowadays supplies most European black truffles (Tuber melanosporum) to markets. Ascospore inoculation has been traditionally used to produce these seedlings, but little scientific information is publicly available on the inoculation methods applied or on the possibility of combining them. We evaluated the potential of sequential inoculation for the controlled colonization of holm oak fine roots by T. melanosporum, with two different nursery assays and a full factorial design. Three inoculation methods were sequentially applied: radicle inoculation, inoculation of the substrate in seedling trays and inoculation of the substrate in the final pot. Despite the differences in the results of the two assays, which suggest that cultivation conditions and/or the timing of nursery operations may influence the relative effectiveness of inoculation methods, the sequential application appeared as an effective and realistic alternative for commercial inoculation of holm oak seedlings with T. melanosporum. The increase in the amount of inoculum applied with each inoculation method improved the mycorrhizal colonization of seedlings, whereas separately none of the inoculation methods appeared clearly superior to the other ones. The depth distribution of truffle mycorrhizae pointed that the inoculation in the final pot was more effective than other methods in lower parts of the root system, whereas the early inoculation appeared more effective to reduce the occurrence of the opportunist ectomycorrhizal fungus Sphaerosporella brunnea. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/EC/H2020/101007623/EU/INnovation in Truffle cultivation, preservAtion, proCessing and wild truffle resources managemenT/INTACT This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 101007623-INTACT info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 2.9 2023 MYCOLOGY 12 / 33 = 0.364 2023 Q2 T2 0.696 2023 Plant Science 2023 Q1 Ecology, Evolution, Behavior and Systematics 2023 Q2 Infectious Diseases 2023 Q2 Genetics 2023 Q3 5.8 2023 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Sánchez, Sergio Universidad de Zaragoza (orcid)0000-0003-4331-9794 Puig-Pey, Meritxell García-Barreda, Sergi Universidad de Zaragoza (orcid)0000-0002-7248-234X 5011 500 Universidad de Zaragoza Dpto. CC.Agrar.y Medio Natural Area Ingeniería Agroforestal 5011 705 Universidad de Zaragoza Dpto. CC.Agrar.y Medio Natural Área Producción Vegetal 127, 10-11 (2023), 1328-1335 FUNGAL BIOLOGY FUNGAL BIOLOGY 1878-6146 528102 http://zaguan.unizar.es/record/170257/files/texto_completo.pdf Postprint 422670 http://zaguan.unizar.es/record/170257/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:170257 articulos driver 2026-04-10-13:45:19 ARTICLE