000170315 001__ 170315
000170315 005__ 20260410165451.0
000170315 0247_ $$2doi$$a10.1128/aem.00225-26
000170315 0248_ $$2sideral$$a148812
000170315 037__ $$aART-2026-148812
000170315 041__ $$aeng
000170315 100__ $$aBenucci, Gian Maria Niccolò
000170315 245__ $$aMycorrhizal competition release and microbial dynamics innative and non-native Tuber melanosporum habitats
000170315 260__ $$c2026
000170315 5060_ $$aAccess copy available to the general public$$fUnrestricted
000170315 5203_ $$aTruffles in the genus Tuber (Pezizales) are among the few ectomycorrhizaltaxa successfully cultivated worldwide. Australia has recently become one of the topproducers of Tuber melanosporum, a high-valued black truffle native to Europe. Truffleco-introductions in the Southern Hemisphere with their Northern Hemisphere plantsymbionts are hypothesized to benefit from reduced ectomycorrhizal competition andnatural enemies. In this study, we tested whether T. melanosporum in Australia experiences competition release by sampling soils and truffles across France, Spain, Italy,and Australia. Fungal and prokaryotic communities in truffle plantations were assessedin native (European) and non-native (Australian) habitats through ITS and 16S rDNAamplicon sequencing from soil and truffles. Community composition was primarilystructured by site of origin and secondarily by presence of brûlé, vegetation-free areainduced by truffle production of plant-growth inhibiting compounds, with significantinteractions indicating site-dependent brûlé effects. European soils showed higherfungal richness outside the brûlé, with higher evenness overall and for ectomycorrhizalfungi only outside brûlé. T. melanosporum showed higher abundance in Australia, withsignificant differences restricted to outside the brûlé. Overall, ectomycorrhizal fungi inEuropean soils had more than four times the taxa and higher diversity compared toAustralian soils. Among the main competitors, Tomentella, Inocybe, and Trichophaeaco-dominated in Europe, versus Scleroderma, Hebeloma, and Tarzetta in Australia. Despitedifferences in soil microbiomes, bacterial communities within T. melanosporum truffleascocarps were strikingly similar across sites and continents and were dominated byBradyrhizobium. Despite high site-level variation, our results support the competitionrelease hypothesis, with reduced enemies benefiting T. melanosporum colonizationoutside the brûlé in Australia. IMPORTANCE: This study provides the first cross-hemisphere analysis of the truffle microbiome, comparing native and non-native soils and truffles from Europeand Australia. We demonstrate that the remarkable success of Tuber melanosporumcultivation in Australia is compatible with ecological release from competitors, whichfavors its development outside the brûlé. At the same time, we reveal strikingcross-hemispheric similarities in truffle-associated bacterial communities, consistentlydominated by Bradyrhizobium. These findings highlight both the novelty and transcontinental relevance of our work, offering new perspectives on fungal ecology and trufflecultivation.
000170315 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000170315 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000170315 700__ $$0(orcid)0000-0002-7248-234X$$aGarcia-Barreda, Sergi
000170315 700__ $$aSanchez, Sergio
000170315 700__ $$0(orcid)0000-0003-3384-7534$$aMarco, Pedro$$uUniversidad de Zaragoza
000170315 700__ $$aDe Miguel, Ana Maria
000170315 700__ $$aLe Tacon, Francois
000170315 700__ $$aMarozzi, Giorgio
000170315 700__ $$aBaciarelli Failini, Leonardo
000170315 700__ $$aEslick, Harry
000170315 700__ $$aElliott, Todd F.
000170315 700__ $$aDeveau, Aurelie
000170315 700__ $$aMurat, Claude
000170315 700__ $$aDonnini, Domizia
000170315 700__ $$aBonito, Gregory
000170315 7102_ $$12008$$2640$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Nutrición Bromatología
000170315 773__ $$g(2026), [21 pp.]$$tApplied and Environmental Microbiology$$x0099-2240
000170315 8564_ $$s4109304$$uhttps://zaguan.unizar.es/record/170315/files/texto_completo.pdf$$yVersión publicada
000170315 8564_ $$s2228500$$uhttps://zaguan.unizar.es/record/170315/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000170315 909CO $$ooai:zaguan.unizar.es:170315$$particulos$$pdriver
000170315 951__ $$a2026-04-10-13:46:25
000170315 980__ $$aARTICLE