000170324 001__ 170324
000170324 005__ 20260410165451.0
000170324 0247_ $$2doi$$a10.1016/bs.mcb.2026.02.013
000170324 0248_ $$2sideral$$a148768
000170324 037__ $$aART-2026-148768
000170324 041__ $$aeng
000170324 100__ $$aCorral-Sancho, María Pilar$$uUniversidad de Zaragoza
000170324 245__ $$aEvaluation of lipid peroxidation as a marker of ferroptosis in colon cancer cells
000170324 260__ $$c2026
000170324 5203_ $$aFerroptosis is a regulated form of cell death driven by iron-dependent lipid peroxidation, with emerging relevance in cancer biology, particularly in therapy-resistant tumors such as colorectal cancer. Accurate and reproducible methods to monitor ferroptosis are essential for understanding its mechanisms and therapeutic potential. In this methodological paper, we present an optimized protocol for the evaluation of lipid peroxidation as a surrogate marker of ferroptosis in colon cancer cell models. Specifically, we describe an approach for quantifying malonylaldehyde and hydroxyalkenals (MDA+ 4-HDA), as key byproducts of lipid oxidation, using a colorimetric assay adapted for cell culture. This methodological framework provides a reliable basis for dissecting ferroptotic responses and for evaluating the activity of ferroptosis-inducing compounds in colorectal cancer research.
000170324 540__ $$9info:eu-repo/semantics/closedAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000170324 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000170324 700__ $$0(orcid)0000-0002-8467-0356$$aBuey, Berta
000170324 700__ $$0(orcid)0000-0001-5573-6144$$aLayunta, Elena$$uUniversidad de Zaragoza
000170324 700__ $$0(orcid)0000-0002-5797-3909$$aLatorre, Eva$$uUniversidad de Zaragoza
000170324 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Anatom.Anatom.Patológ.Com
000170324 7102_ $$11002$$2050$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Biología Celular
000170324 773__ $$pMethods cell biol.$$tMETHODS IN CELL BIOLOGY$$x0091-679X
000170324 8564_ $$s417761$$uhttps://zaguan.unizar.es/record/170324/files/texto_completo.pdf$$yPostprint
000170324 8564_ $$s890025$$uhttps://zaguan.unizar.es/record/170324/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000170324 909CO $$ooai:zaguan.unizar.es:170324$$particulos$$pdriver
000170324 951__ $$a2026-04-10-13:46:35
000170324 980__ $$aARTICLE