170324 20260410165451.0 doi 10.1016/bs.mcb.2026.02.013 sideral 148768 ART-2026-148768 eng Corral-Sancho, María Pilar Universidad de Zaragoza Evaluation of lipid peroxidation as a marker of ferroptosis in colon cancer cells 2026 Ferroptosis is a regulated form of cell death driven by iron-dependent lipid peroxidation, with emerging relevance in cancer biology, particularly in therapy-resistant tumors such as colorectal cancer. Accurate and reproducible methods to monitor ferroptosis are essential for understanding its mechanisms and therapeutic potential. In this methodological paper, we present an optimized protocol for the evaluation of lipid peroxidation as a surrogate marker of ferroptosis in colon cancer cell models. Specifically, we describe an approach for quantifying malonylaldehyde and hydroxyalkenals (MDA+ 4-HDA), as key byproducts of lipid oxidation, using a colorimetric assay adapted for cell culture. This methodological framework provides a reliable basis for dissecting ferroptotic responses and for evaluating the activity of ferroptosis-inducing compounds in colorectal cancer research. info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Buey, Berta (orcid)0000-0002-8467-0356 Layunta, Elena Universidad de Zaragoza (orcid)0000-0001-5573-6144 Latorre, Eva Universidad de Zaragoza (orcid)0000-0002-5797-3909 1001 025 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Anatom.Anatom.Patológ.Com 1002 050 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Biología Celular Methods cell biol. METHODS IN CELL BIOLOGY 0091-679X 417761 http://zaguan.unizar.es/record/170324/files/texto_completo.pdf Postprint 890025 http://zaguan.unizar.es/record/170324/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:170324 articulos driver 2026-04-10-13:46:35 ARTICLE