000170325 001__ 170325
000170325 005__ 20260410165451.0
000170325 0247_ $$2doi$$a10.1186/s40793-025-00848-6
000170325 0248_ $$2sideral$$a148775
000170325 037__ $$aART-2026-148775
000170325 041__ $$aeng
000170325 100__ $$0(orcid)0000-0003-3384-7534$$aMarco, Pedro$$uUniversidad de Zaragoza
000170325 245__ $$aBacterial communities show distinctive spatial diversity patterns in productive truffle orchards amended with peat-based substrate
000170325 260__ $$c2026
000170325 5060_ $$aAccess copy available to the general public$$fUnrestricted
000170325 5203_ $$aAs truffle cultivation expands, growers empirically develop new agronomic management practices aimed at promoting truffle growth such as “truffle nests”, localized peat amendments that are supplemented with truffle spore inoculum. Previous research showed that nests contain lower fungal diversity than the surrounding soil, which could encourage its occupation by pioneer species such as Tuber melanosporum. However, truffle nests did not quickly stimulate truffle mycelium growth. We hypothesized that the bacterial community from the soil may be the first to colonize nests and that fungal and bacterial diversity in nests would have an inverse relationship. To test this, we characterized the bacterial community of truffle nests, via 16S rRNA gene amplicon sequencing, in two orchards during the two years after establishing the nests. Unexpectedly, we did not find drastic differences in the bacterial diversity inside nests with respect to the bulk soil or the commercial substrate before being introduced in the field. However, Proteobacteria richness in nests was positively correlated to truffle mycelium abundance, which together with a higher relative abundance of Proteobacteria in nests than in bulk soil, indicates a possible underlying factor for the performance of nests in truffle plantations. Fungal and bacterial richness was positively correlated in nests, countering our hypothesis that bacterial diversity would negatively impact fungal diversity.
000170325 536__ $$9info:eu-repo/grantAgreement/ES/INIA/RTA2015-00053-00-00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2022-139407OR-I00
000170325 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000170325 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000170325 700__ $$0(orcid)0000-0003-4331-9794$$aSánchez, Sergio
000170325 700__ $$0(orcid)0000-0002-7248-234X$$aGarcia-Barreda, Sergi
000170325 700__ $$aParladé, Javier
000170325 700__ $$aRondolini, Mara
000170325 700__ $$aGonzález, Vicente
000170325 700__ $$aBenucci, Gian Maria Niccolò
000170325 700__ $$aBonito, Gregory
000170325 7102_ $$12008$$2640$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Nutrición Bromatología
000170325 773__ $$g21, 26 (2026), [15 pp.]$$pEnviron. Microbiome$$tEnvironmental Microbiome$$x2524-6372
000170325 787__ $$t16S rRNA gene amplicon sequencing data$$tITS amplicon sequencing data$$tTuber melanosporum mycelium qPCR data$$tData sets and R code to reproduce the analyses in the study$$whttps://www.ncbi.nlm.nih.gov/bioprojec t/PRJNA1255533$$whttps://www.ncbi.nlm.nih.gov/bi oproject/PRJNA938598$$whttps://doi.org/10.6084/m9.figshare.2989087$$whttps://github.com/Gian77/Published-R-Code/tree/master /Garcia-Barreda_et_al_TruffleNestBacteria
000170325 8564_ $$s4365196$$uhttps://zaguan.unizar.es/record/170325/files/texto_completo.pdf$$yVersión publicada
000170325 8564_ $$s2183189$$uhttps://zaguan.unizar.es/record/170325/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000170325 909CO $$ooai:zaguan.unizar.es:170325$$particulos$$pdriver
000170325 951__ $$a2026-04-10-13:46:36
000170325 980__ $$aARTICLE