170325 20260410165451.0 doi 10.1186/s40793-025-00848-6 sideral 148775 ART-2026-148775 eng Marco, Pedro Universidad de Zaragoza (orcid)0000-0003-3384-7534 Bacterial communities show distinctive spatial diversity patterns in productive truffle orchards amended with peat-based substrate 2026 As truffle cultivation expands, growers empirically develop new agronomic management practices aimed at promoting truffle growth such as “truffle nests”, localized peat amendments that are supplemented with truffle spore inoculum. Previous research showed that nests contain lower fungal diversity than the surrounding soil, which could encourage its occupation by pioneer species such as Tuber melanosporum. However, truffle nests did not quickly stimulate truffle mycelium growth. We hypothesized that the bacterial community from the soil may be the first to colonize nests and that fungal and bacterial diversity in nests would have an inverse relationship. To test this, we characterized the bacterial community of truffle nests, via 16S rRNA gene amplicon sequencing, in two orchards during the two years after establishing the nests. Unexpectedly, we did not find drastic differences in the bacterial diversity inside nests with respect to the bulk soil or the commercial substrate before being introduced in the field. However, Proteobacteria richness in nests was positively correlated to truffle mycelium abundance, which together with a higher relative abundance of Proteobacteria in nests than in bulk soil, indicates a possible underlying factor for the performance of nests in truffle plantations. Fungal and bacterial richness was positively correlated in nests, countering our hypothesis that bacterial diversity would negatively impact fungal diversity. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/INIA/RTA2015-00053-00-00 info:eu-repo/grantAgreement/ES/MICINN/PID2022-139407OR-I00 info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Sánchez, Sergio (orcid)0000-0003-4331-9794 Garcia-Barreda, Sergi (orcid)0000-0002-7248-234X Parladé, Javier Rondolini, Mara González, Vicente Benucci, Gian Maria Niccolò Bonito, Gregory 2008 640 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Nutrición Bromatología 21, 26 (2026), [15 pp.] Environ. Microbiome Environmental Microbiome 2524-6372 16S rRNA gene amplicon sequencing data https://www.ncbi.nlm.nih.gov/bioprojec t/PRJNA1255533 ITS amplicon sequencing data https://www.ncbi.nlm.nih.gov/bi oproject/PRJNA938598 Tuber melanosporum mycelium qPCR data https://doi.org/10.6084/m9.figshare.2989087 Data sets and R code to reproduce the analyses in the study https://github.com/Gian77/Published-R-Code/tree/master /Garcia-Barreda_et_al_TruffleNestBacteria 4365196 http://zaguan.unizar.es/record/170325/files/texto_completo.pdf Versión publicada 2183189 http://zaguan.unizar.es/record/170325/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:170325 articulos driver 2026-04-10-13:46:36 ARTICLE