Cam β-NGf supplementation: a promising approach to preserve dromedary camel sperm quality during short-term storage

Doghbri, Lamia ; Atigui, Moufida ; Salhi, Imed ; Carvajal-Serna, Melissa ; Casao, Adriana (Universidad de Zaragoza) ; Dbara, Mohamed ; Pérez Pe, Rosaura María (Universidad de Zaragoza) ; Hammadi, Mohamed ; Kdidi, Samia

Resumen: Introduction
The role of camel βeta-Nerve Growth Factor protein (Cam β-NGF), isolated from camel seminal plasma, in preserving sperm quality remains poorly defined. This study aimed to evaluate the impact of Cam β-NGF on mass motility, viability, and membrane integrity in dromedary (Camelus dromedarius) semen stored for 24 hours at 4 °C.

Methods
The semen samples were treated with varying concentrations of Cam β-NGF (10, 100, 500, and 1000 ng/mL) after preservation. Ten semen samples with mass motility ≥ 3 and viability ≥ 60% were selected, washed, suspended in HBSS, and treated with TCF-EY. After 24 hours of storage at 4 °C, samples were maintained at 36 °C with Cam β-NGF supplementation for 60 minutes. Mass motility, viability, and membrane integrity were assessed at T0, T30, and T60 minutes.

Results
The results revealed a significant decline in sperm quality traits after 24 hours of storage at 4 °C. However, mass motility was conserved after 30 minutes of incubation with 1000 ng/mL and 500 ng/mL of Cam β-NGF (p < 0.05). On the other hand, viability was significantly higher after 60 minutes of incubation with 1000, 500, and 100 ng/mL of Cam β-NGF compared to the control extender and 10 ng/mL supplementation (p < 0.05). Additionally, membrane integrity was significantly maintained at T30 and T60 minutes with 1000, 500, and 100 ng/mL of Cam β-NGF (p < 0.05).

Discussion
Short‐term storage at 4 °C markedly reduced dromedary camel sperm motility, viability, and membrane integrity, consistent with cold‐induced oxidative stress. Adding purified Cam β-NGF dose-dependently preserved these parameters over 1 h of incubation, indicating its value as a protective supplement for semen storage.

Conclusion
Cam β-NGF helps protect and maintain sperm traits during short-term storage; it seems to reduce alterations that might lower sperm quality. Thus, supplementing semen with Cam β-NGF may help maintain sperm quality, offering potential benefits for artificial insemination programs in dromedaries.

Idioma: Inglés

DOI: 10.2174/0118740707389100251014053002

Año: 2025

Publicado en: Open Biotechnology Journal 19 (2025), e18740707389100 [9 p.]
ISSN: 1874-0707

Financiación: info:eu-repo/grantAgreement/ES/DGA/A07-17R

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Zoología (Dpto. Bioq.Biolog.Mol. Celular)