170379 20260420103354.0 doi 10.2174/0118740707389100251014053002 sideral 148919 ART-2025-148919 eng Doghbri, Lamia Cam β-NGf supplementation: a promising approach to preserve dromedary camel sperm quality during short-term storage 2025 Introduction The role of camel βeta-Nerve Growth Factor protein (Cam β-NGF), isolated from camel seminal plasma, in preserving sperm quality remains poorly defined. This study aimed to evaluate the impact of Cam β-NGF on mass motility, viability, and membrane integrity in dromedary (Camelus dromedarius) semen stored for 24 hours at 4 °C. Methods The semen samples were treated with varying concentrations of Cam β-NGF (10, 100, 500, and 1000 ng/mL) after preservation. Ten semen samples with mass motility ≥ 3 and viability ≥ 60% were selected, washed, suspended in HBSS, and treated with TCF-EY. After 24 hours of storage at 4 °C, samples were maintained at 36 °C with Cam β-NGF supplementation for 60 minutes. Mass motility, viability, and membrane integrity were assessed at T0, T30, and T60 minutes. Results The results revealed a significant decline in sperm quality traits after 24 hours of storage at 4 °C. However, mass motility was conserved after 30 minutes of incubation with 1000 ng/mL and 500 ng/mL of Cam β-NGF (p < 0.05). On the other hand, viability was significantly higher after 60 minutes of incubation with 1000, 500, and 100 ng/mL of Cam β-NGF compared to the control extender and 10 ng/mL supplementation (p < 0.05). Additionally, membrane integrity was significantly maintained at T30 and T60 minutes with 1000, 500, and 100 ng/mL of Cam β-NGF (p < 0.05). Discussion Short‐term storage at 4 °C markedly reduced dromedary camel sperm motility, viability, and membrane integrity, consistent with cold‐induced oxidative stress. Adding purified Cam β-NGF dose-dependently preserved these parameters over 1 h of incubation, indicating its value as a protective supplement for semen storage. Conclusion Cam β-NGF helps protect and maintain sperm traits during short-term storage; it seems to reduce alterations that might lower sperm quality. Thus, supplementing semen with Cam β-NGF may help maintain sperm quality, offering potential benefits for artificial insemination programs in dromedaries. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/DGA/A07-17R info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Atigui, Moufida Salhi, Imed Carvajal-Serna, Melissa (orcid)0000-0003-3929-5064 Casao, Adriana Universidad de Zaragoza (orcid)0000-0003-1997-4262 Dbara, Mohamed Pérez Pe, Rosaura María Universidad de Zaragoza (orcid)0000-0002-2312-6402 Hammadi, Mohamed Kdidi, Samia 1002 819 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Zoología 19 (2025), e18740707389100 [9 p.] Open Biotechnology Journal 1874-0707 1552993 http://zaguan.unizar.es/record/170379/files/texto_completo.pdf Versión publicada 2300594 http://zaguan.unizar.es/record/170379/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:170379 articulos driver 2026-04-18-10:48:23 ARTICLE