000170392 001__ 170392 000170392 005__ 20260420103354.0 000170392 0247_ $$2doi$$a10.1007/s10096-012-1544-7 000170392 0248_ $$2sideral$$a77444 000170392 037__ $$aART-2012-77444 000170392 041__ $$aeng 000170392 100__ $$0(orcid)0000-0003-0765-7227$$aGoñi, P.$$uUniversidad de Zaragoza 000170392 245__ $$aEvaluation of an immunochromatographic dip strip test for simultaneous detection of Cryptosporidium spp, Giardia duodenalis, and Entamoeba histolytica antigens in human faecal samples 000170392 260__ $$c2012 000170392 5203_ $$aImmunochromatographic (IC) tests may play an important role in the future diagnosis of parasitic diseases because of their speed and simplicity of use. A recently developed test to detect Cryptosporidium spp, Giardia duodenalis and Entamoeba histolytica was evaluated. Microscopy and PCR were the “gold standard” reference techniques and the results of this IC test were compared with those obtained with ELISA and IC single test for the three parasites. One hundred sixty stool samples were assayed. Using microscopy, 22 samples were diagnosed as positive for Cryptosporidium spp., 31 for Giardia duodenalis, 41 for Entamoeba histolytica/dispar, and 68 had a negative diagnosis for the three parasites. Results of IC tests show sensitivities of 70–72% for Cryptosporidium, 90–97% for Giardia and 62.5% for Entamoeba histolytica. Specificities were of 93.6–94.9%, >99% and 96.1%, respectively. In all diagnoses, agreement with microscopy and PCR was over 90%, except in the triple test and microscopy in E. histolytica detection that was 76.3%, due to the inability of microscopy to differentiate E. histolytica from nonpathogenic species such as E. dispar or E. moshkovskii. The triple stool immunoassays provide adequate sensitivities and specificities for use in outbreak situations, for screening proposals and for massive assays in endemic areas where a large number of samples must be analysed or as complementary test for individual diagnosis. 000170392 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B-8 000170392 540__ $$9info:eu-repo/semantics/closedAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000170392 590__ $$a3.024$$b2012 000170392 591__ $$aMICROBIOLOGY$$b43 / 116 = 0.371$$c2012$$dQ2$$eT2 000170392 591__ $$aINFECTIOUS DISEASES$$b25 / 70 = 0.357$$c2012$$dQ2$$eT2 000170392 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000170392 700__ $$aMartín, B. 000170392 700__ $$0(orcid)0000-0002-1360-8686$$aVillacampa, M. 000170392 700__ $$aGarcía, A. 000170392 700__ $$0(orcid)0000-0002-9742-1463$$aSeral, C.$$uUniversidad de Zaragoza 000170392 700__ $$0(orcid)0000-0002-2519-701X$$aCastillo, F.J.$$uUniversidad de Zaragoza 000170392 700__ $$0(orcid)0000-0001-5829-9787$$aClavel, A.$$uUniversidad de Zaragoza 000170392 7102_ $$11008$$2660$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Parasitología 000170392 7102_ $$11008$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Microbiología 000170392 773__ $$g31, 8 (2012), 2077-2082$$pEur. j. clin. microbiol. infect. dis.$$tEUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES$$x0934-9723 000170392 8564_ $$s158621$$uhttps://zaguan.unizar.es/record/170392/files/texto_completo.pdf$$yVersión publicada 000170392 8564_ $$s2149119$$uhttps://zaguan.unizar.es/record/170392/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000170392 909CO $$ooai:zaguan.unizar.es:170392$$particulos$$pdriver 000170392 951__ $$a2026-04-18-10:48:41 000170392 980__ $$aARTICLE