170392 20260420103354.0 doi 10.1007/s10096-012-1544-7 sideral 77444 ART-2012-77444 eng Goñi, P. Universidad de Zaragoza (orcid)0000-0003-0765-7227 Evaluation of an immunochromatographic dip strip test for simultaneous detection of Cryptosporidium spp, Giardia duodenalis, and Entamoeba histolytica antigens in human faecal samples 2012 Immunochromatographic (IC) tests may play an important role in the future diagnosis of parasitic diseases because of their speed and simplicity of use. A recently developed test to detect Cryptosporidium spp, Giardia duodenalis and Entamoeba histolytica was evaluated. Microscopy and PCR were the “gold standard” reference techniques and the results of this IC test were compared with those obtained with ELISA and IC single test for the three parasites. One hundred sixty stool samples were assayed. Using microscopy, 22 samples were diagnosed as positive for Cryptosporidium spp., 31 for Giardia duodenalis, 41 for Entamoeba histolytica/dispar, and 68 had a negative diagnosis for the three parasites. Results of IC tests show sensitivities of 70–72% for Cryptosporidium, 90–97% for Giardia and 62.5% for Entamoeba histolytica. Specificities were of 93.6–94.9%, >99% and 96.1%, respectively. In all diagnoses, agreement with microscopy and PCR was over 90%, except in the triple test and microscopy in E. histolytica detection that was 76.3%, due to the inability of microscopy to differentiate E. histolytica from nonpathogenic species such as E. dispar or E. moshkovskii. The triple stool immunoassays provide adequate sensitivities and specificities for use in outbreak situations, for screening proposals and for massive assays in endemic areas where a large number of samples must be analysed or as complementary test for individual diagnosis. info:eu-repo/grantAgreement/ES/DGA/B-8 info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 3.024 2012 MICROBIOLOGY 43 / 116 = 0.371 2012 Q2 T2 INFECTIOUS DISEASES 25 / 70 = 0.357 2012 Q2 T2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Martín, B. Villacampa, M. (orcid)0000-0002-1360-8686 García, A. Seral, C. Universidad de Zaragoza (orcid)0000-0002-9742-1463 Castillo, F.J. Universidad de Zaragoza (orcid)0000-0002-2519-701X Clavel, A. Universidad de Zaragoza (orcid)0000-0001-5829-9787 1008 660 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Parasitología 1008 630 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Microbiología 31, 8 (2012), 2077-2082 Eur. j. clin. microbiol. infect. dis. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES 0934-9723 158621 http://zaguan.unizar.es/record/170392/files/texto_completo.pdf Versión publicada 2149119 http://zaguan.unizar.es/record/170392/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:170392 articulos driver 2026-04-18-10:48:41 ARTICLE