170463 20260422085545.0 doi 10.3390/antibiotics15030321 sideral 148979 ART-2026-148979 eng Ezquerra-Aznárez, José Manuel Universidad de Zaragoza (orcid)0000-0001-9771-4912 New Chemical Scaffold with Antimicrobial Activity Identified in a Screening of Industrial Photoactive Compounds 2026 Background/Objectives: The emergence of antimicrobial resistance threatens advances achieved by medicine in the last century. This situation has been exacerbated by the suboptimal outcome of screening campaigns to provide novel antimicrobials. Methods: An alternative strategy was employed to identify new chemical scaffolds with antimicrobial activity. A collection of photoactive compounds originally synthesized for industrial purposes was screened for antibacterial activity. Results: 4H-pyran-4-ylidenes were identified as active against Gram-positive bacteria. Compounds belonging to this family displayed dose-dependent bactericidal activity against both wild-type and methicillin-resistant Staphylococcus aureus. No cytotoxicity was observed in the HepG2 hepatic cell line at the concentrations required for antimicrobial activity against S. aureus. Resistance to 4H-pyran-4-ylidenes in S. aureus was associated with point mutations in the rny locus, which encodes for a ribonuclease that plays a key role in RNA homeostasis. Conclusions: These findings indicate that chemical libraries not originally intended for drug discovery can be an innovative source of chemical diversity for the development of novel antimicrobials. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/DGA/E47-23R info:eu-repo/grantAgreement/ES/UZ/UZ2023-CIE-01 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Alonso-Román, Raquel Lucía, Ainhoa Universidad de Zaragoza (orcid)0000-0002-0111-4697 Andreu, Raquel Universidad de Zaragoza (orcid)0000-0002-3206-9868 Franco, Santiago Universidad de Zaragoza (orcid)0000-0001-9747-1789 Aínsa, José A. Universidad de Zaragoza (orcid)0000-0003-2076-844X Ramón-García, Santiago (orcid)0000-0002-8480-0325 1011 630 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Microbiología 2013 765 Universidad de Zaragoza Dpto. Química Orgánica Área Química Orgánica 15, 3 (2026), 321 [17 pp.] Antibiotics Antibiotics 2079-6382 1373292 http://zaguan.unizar.es/record/170463/files/texto_completo.pdf Versión publicada 2340306 http://zaguan.unizar.es/record/170463/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:170463 articulos driver 2026-04-22-08:32:56 ARTICLE