000171187 001__ 171187
000171187 005__ 20260515163945.0
000171187 0247_ $$2doi$$a10.3390/v18040462
000171187 0248_ $$2sideral$$a149251
000171187 037__ $$aART-2026-149251
000171187 041__ $$aeng
000171187 100__ $$0(orcid)0000-0002-9723-9004$$aGómez, Álex$$uUniversidad de Zaragoza
000171187 245__ $$aIn Vitro Antiviral Properties of Two Recombinant Sendai Virus Vectors Encoding ORFV 011 and ORFV 059 Genes
000171187 260__ $$c2026
000171187 5060_ $$aAccess copy available to the general public$$fUnrestricted
000171187 5203_ $$aOrf virus (ORFV) is a globally distributed zoonotic parapoxvirus that causes a highly contagious mucocutaneous disease in small ruminants. Despite the urgent demand for vaccination-based control, no licensed vaccines are currently available universally. In this study, we generated two recombinant Sendai virus (SeV) vectors expressing ORFV 011 (rSeV-GFP-B2L) and ORFV 059 (rSeV-GFP-059) genes and evaluated their ability to stimulate antiviral responses in vitro. Following the transduction, we assessed transgene expression, innate immune activation, induction of interferon-stimulated genes (A3Z1, OBST2, SAMHD1), and antiviral activity. Both vectors significantly upregulated pattern recognition receptors (TLRs, RIG-I) and type I interferon (IFN-β) genes, with rSeV-GFP-059 inducing the strongest response. Remarkably, OBST2 was robustly upregulated, suggesting a potential role in restricting ORFV replication. Antiviral activity assays revealed a marked reduction in ORFV DNA copies and a mild decrease in ORFV RNA transcription in rSeV-GFP-059-transduced cells, particularly at later time points, accompanied by complete abrogation of the typical cytopathic effect. Collectively, these results demonstrate that SeV-based vectors, particularly rSeV-GFP-059, efficiently prime antiviral immunity and suppress ORFV replication, establishing a promising platform for further in vivo vaccine evaluation in sheep.
000171187 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000171187 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000171187 700__ $$aGlaria, Idoia
000171187 700__ $$aMoncayola, Irati
000171187 700__ $$aPuzol, Leonor
000171187 700__ $$aArriazu, Laura
000171187 700__ $$aCalero, Ainhoa
000171187 700__ $$0(orcid)0000-0002-1204-4356$$ade Blas, Ignacio$$uUniversidad de Zaragoza
000171187 700__ $$aNazábal, Mikel
000171187 700__ $$aHualde, Itziar
000171187 700__ $$aLee, Benhur
000171187 700__ $$0(orcid)0000-0002-2053-9842$$aLuján, Lluís$$uUniversidad de Zaragoza
000171187 700__ $$aAmann, Ralf
000171187 700__ $$aEcheverría, Irache
000171187 700__ $$aReina, Ramsés
000171187 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000171187 773__ $$g18, 4 (2026), 462 [17 pp.]$$pVIRUSES-BASEL$$tVIRUSES-BASEL$$x1999-4915
000171187 8564_ $$s2349123$$uhttps://zaguan.unizar.es/record/171187/files/texto_completo.pdf$$yVersión publicada
000171187 8564_ $$s2387902$$uhttps://zaguan.unizar.es/record/171187/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000171187 909CO $$ooai:zaguan.unizar.es:171187$$particulos$$pdriver
000171187 951__ $$a2026-05-15-14:54:40
000171187 980__ $$aARTICLE