000171237 001__ 171237
000171237 005__ 20260515163946.0
000171237 0247_ $$2doi$$a10.3390/microorganisms14040805
000171237 0248_ $$2sideral$$a149210
000171237 037__ $$aART-2026-149210
000171237 041__ $$aeng
000171237 100__ $$0(orcid)0009-0008-8782-397X$$aGómara, Paula$$uUniversidad de Zaragoza
000171237 245__ $$aRole of stress response genes in resistance and germination of Bacillus Subtilis spores
000171237 260__ $$c2026
000171237 5060_ $$aAccess copy available to the general public$$fUnrestricted
000171237 5203_ $$aBacterial adaptation to hostile environments depends on the coordinated expression of stress response genes. When adverse conditions persist and nutrients become limiting, sporulating species may initiate sporulation as a last-resort survival strategy. However, sporulation under such conditions may alter the resistance and germination properties of the resulting spores. In this study, we investigated whether stress response regulators that facilitate vegetative cell adaptation to temperature and/or salinity changes during growth can influence the properties of Bacillus subtilis 168 spores. To this end, we examined the resistance and germination of mutant spores lacking key regulators of stress response pathways (SigB, SigW, SigX, Fur, HrcA, CtsR, and CssRS regulon), all produced under optimal sporulation conditions. The constitutive activation of the SigB-mediated general stress response, achieved through the deletion of its negative regulator RsbX, reduced spore heat resistance by 2.2-fold compared to the parental strain, while no effect was observed in vegetative cells. Additionally, ΔrsbX spores displayed both impaired nutrient-induced and CaDPA-induced germination. Collectively, these findings suggest that stress response regulators can influence spore behavior, although their effects may differ from those observed in vegetative cells.
000171237 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/PID2019-104712RA-I00
000171237 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000171237 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000171237 700__ $$aPinilla, Emma
000171237 700__ $$aBellón, Jorge$$uUniversidad de Zaragoza
000171237 700__ $$0(orcid)0000-0002-5895-2157$$aGayán, Elisa$$uUniversidad de Zaragoza
000171237 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000171237 773__ $$g14, 4 (2026), 805$$pMicroorganisms$$tMicroorganisms$$x2076-2607
000171237 8564_ $$s851676$$uhttps://zaguan.unizar.es/record/171237/files/texto_completo.pdf$$yVersión publicada
000171237 8564_ $$s2362028$$uhttps://zaguan.unizar.es/record/171237/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000171237 909CO $$ooai:zaguan.unizar.es:171237$$particulos$$pdriver
000171237 951__ $$a2026-05-15-14:55:42
000171237 980__ $$aARTICLE