171617 20260527123126.0 doi 10.1021/acsfoodscitech.5c01241 sideral 149405 ART-2026-149405 eng Pihen, Christelle Pulsed Electric Fields as a Cutting-Edge Solution to Ensure the Safety of Pineapple Juice: Targeting Escherichia coli O157:H7 and Listeria monocytogenes 5672 2026 This study evaluates the effectiveness of pulsed electric field (PEF) technology for pasteurizing pineapple juice to inactivate Escherichia coli O157:H7 and Listeria monocytogenes 5672. Pineapple juice was treated with PEF pulses of 5 μs at electric field intensities of 15, 20, and 25 kV/cm, with frequencies ranging from 8 to 44 Hz. Treatment durations ranged from 12 to 197 s, corresponding to energy levels between 18 and 192 kJ/kg, while exit temperatures were controlled between 40 and 70 °C. Results showed that higher exit temperatures and electric field strengths significantly enhanced the inactivation of E. coli O157:H7, with the most effective treatment being at 70 °C and 25 kV/cm, achieving ≥3-log reductions. For L. monocytogenes 5672, reductions of 4–5 log cycles were observed under most conditions, except at 15 kV/cm, where a 2-log reduction was noted. After treatment, all samples were incubated for 72 h under refrigeration; E. coli O157:H7 showed an additional lethal effect during storage. This leads to reductions exceeding 5 logs for those juices treated at 20 and 25 kV/cm, while L. monocytogenes 5672 showed less significant changes. These findings highlight the potential of PEF technology for enhancing food safety in juice preservation. Access copy available to the general public Unrestricted info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Sanz, Jorge Berzosa, Alejandro Universidad de Zaragoza Raso, Javier Universidad de Zaragoza (orcid)0000-0003-3957-9091 Lopez-Malo, Aurelio Ramirez-Corona, Nelly 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos (2026), [13 pp.] ACS Food Science and Technology 2692-1944 4317522 http://zaguan.unizar.es/record/171617/files/texto_completo.pdf Versión publicada 3058821 http://zaguan.unizar.es/record/171617/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:171617 articulos driver 2026-05-27-11:25:02 ARTICLE