000032153 001__ 32153
000032153 005__ 20170504093354.0
000032153 0247_ $$2doi$$a10.1371/journal.pone.0107393
000032153 0248_ $$2sideral$$a91021
000032153 037__ $$aART-2014-91021
000032153 041__ $$aeng
000032153 100__ $$aElosegui-Artola, A.
000032153 245__ $$aImage analysis for the quantitative comparison of stress fibers and focal adhesions
000032153 260__ $$c2014
000032153 5060_ $$aAccess copy available to the general public$$fUnrestricted
000032153 5203_ $$aActin stress fibers (SFs) detect and transmit forces to the extracellular matrix through focal adhesions (FAs), and molecules in this pathway determine cellular behavior. Here, we designed two different computational tools to quantify actin SFs and the distribution of actin cytoskeletal proteins within a normalized cellular morphology. Moreover, a systematic cell response comparison between the control cells and those with impaired actin cytoskeleton polymerization was performed to demonstrate the reliability of the tools. Indeed, a variety of proteins that were present within the string beginning at the focal adhesions (vinculin) up to the actin SFs contraction (non-muscle myosin II (NMMII)) were analyzed. Finally, the software used allows for the quantification of the SFs based on the relative positions of FAs. Therefore, it provides a better insight into the cell mechanics and broadens the knowledge of the nature of SFs.
000032153 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/DPI2012-38090-C03-01$$9info:eu-repo/grantAgreement/ES/MICINN/ISCIII-PS09-00393$$9info:eu-repo/grantAgreement/EUR/FP7/ERC2012-StG-306751
000032153 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000032153 590__ $$a3.234$$b2014
000032153 591__ $$aMULTIDISCIPLINARY SCIENCES$$b9 / 57 = 0.158$$c2014$$dQ1$$eT1
000032153 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000032153 700__ $$aJorge-Peñas, A.
000032153 700__ $$0(orcid)0000-0001-7808-381X$$aMoreno-Arotzena, O.
000032153 700__ $$aOregi, A.
000032153 700__ $$aLasa, M.
000032153 700__ $$0(orcid)0000-0002-9864-7683$$aGarcía-Aznar, J.M.$$uUniversidad de Zaragoza
000032153 700__ $$aDe Juan-Pardo, E.M.
000032153 700__ $$aAldabe, R.
000032153 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDepartamento de Ingeniería Mecánica$$cMec. de Medios Contínuos y Teor. de Estructuras
000032153 773__ $$g9, 9 (2014), 1-10$$pPLoS One$$tPLoS One$$x1932-6203
000032153 8564_ $$s2721729$$uhttps://zaguan.unizar.es/record/32153/files/texto_completo.pdf$$yVersión publicada
000032153 8564_ $$s119323$$uhttps://zaguan.unizar.es/record/32153/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000032153 909CO $$ooai:zaguan.unizar.es:32153$$particulos$$pdriver
000032153 951__ $$a2017-05-04-09:32:42
000032153 980__ $$aARTICLE