32169 20210618112019.0 doi 10.1186/1471-2180-14-21 sideral 85206 ART-2014-85206 eng Cabal, A. Single nucleotide polymorphism (SNP) analysis used for the phylogeny of the Mycobacterium tuberculosis complex based on a pyrosequencing assay 2014 Access copy available to the general public Unrestricted Background Different polymorphisms have been described as markers to classify the lineages of the Mycobacterium tuberculosis complex. The analysis of nine single nucleotide polymorphisms (SNPs) was used to describe seven SNPs cluster groups (SCGs). We attempted to classify those strains that could not been categorized into lineages by the genotyping methods used in the routine testing. Results The M. tuberculosis complex isolates collected in 2010 in our region were analysed. A new method based on multiplex-PCRs and pyrosequencing to analyse these SNPs was designed. For the pyrosequencing assay nine SNPs that defined the seven SCGs were selected from the literature: 1977, 74092, 105139, 232574, 311613, 913274, 2460626, 3352929 and gyrA95. In addition, SNPs in katG463, mgtC182, Ag85C103 and RDRio deletion were detected. info:eu-repo/grantAgreement/ES/FIS/FIS09-051 info:eu-repo/grantAgreement/ES/FIS/FIS12/1970 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 2.729 2014 MICROBIOLOGY 51 / 119 = 0.429 2014 Q2 T2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Strunk, M. Domínguez, J. Lezcano, M.A. Vitoria, M.A. Universidad de Zaragoza (orcid)0000-0003-4198-6622 Ferrero, M. Martín, C. Iglesias, M.J. Universidad de Zaragoza (orcid)0000-0003-2266-8602 Samper, S. 1008 630 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Microbiología 1008 615 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Medic.Prevent.Salud Públ. 14, 21 (2014), [9 pp.] BMC Microbiol. BMC MICROBIOLOGY 1471-2180 934224 http://zaguan.unizar.es/record/32169/files/texto_completo.pdf Versión publicada 106323 http://zaguan.unizar.es/record/32169/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:32169 articulos driver 2021-06-18-11:14:21 ARTICLE