000032199 001__ 32199
000032199 005__ 20170327111908.0
000032199 0247_ $$2doi$$a10.1186/s12866-015-0524-3
000032199 0248_ $$2sideral$$a92337
000032199 037__ $$aART-2015-92337
000032199 041__ $$aeng
000032199 100__ $$aSoria-Lozano, P.
000032199 245__ $$aIn vitro effect photodynamic therapy with differents photosensitizers on cariogenic microorganisms
000032199 260__ $$c2015
000032199 5060_ $$aAccess copy available to the general public$$fUnrestricted
000032199 5203_ $$aBackground: Antimicrobial photodynamic therapy has been proposed as an alternative to suppress subgingival species. This results from the balance among Streptococcus sanguis, Streptococcus mutans and Candida albicans in the dental biofilm. Not all the photosensitizers have the same photodynamic effect against the different microorganims. The objective of this study is to compare in vitro the photodynamic effect of methylene blue (MB), rose Bengal (RB) and curcumin (CUR) in combination with white light on the cariogenic microorganism S. mutans, S. sanguis and C. albicans. Results: Photodynamic therapy with MB, RB and CUR inhibited 6 log 10 the growth of both bacteria but at different concentrations: 0.31-0.62 µg/ml and 0.62-1.25 µg/ml RB were needed to photoinactivate S. mutans and S. sanguis, respectively//1.25-2.5 µg/ml MB for both species//whereas higher CUR concentrations (80-160 µg/ml and 160-320 µg/ml) were required to obtain the same reduction in S. mutans and S. sanguis viability respectively. The minimal fungicidal concentration of MB for 5 log10 CFU reduction (4.5 McFarland) was 80-160 µg/ml, whereas for RB it ranged between 320 and 640 µg/ml. For CUR, even the maximum studied concentration (1280 µg/ml) did not reach that inhibition. Incubation time had no effect in all experiments. Conclusions: Photodynamic therapy with RB, MB and CUR and white light is effective in killing S. mutans and S. sanguis strains, although MB and RB are more efficient than CUR. C. albicans required higher concentrations of all photosensitizers to obtain a fungicidal effect, being MB the most efficient and CUR ineffective.
000032199 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/CTQ2013-48767-C3-2-R
000032199 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000032199 590__ $$a2.581$$b2015
000032199 591__ $$aMICROBIOLOGY$$b58 / 123 = 0.472$$c2015$$dQ2$$eT2
000032199 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000032199 700__ $$aGilaberte, Y.
000032199 700__ $$aPaz-Cristobal, M.
000032199 700__ $$aPérez-Artiaga, L.
000032199 700__ $$aLampaya-Pérez, V.
000032199 700__ $$0(orcid)0000-0003-2968-228X$$aAporta, J.$$uUniversidad de Zaragoza
000032199 700__ $$aPérez-Laguna, V.
000032199 700__ $$aGarcía-Luque, I.
000032199 700__ $$aRevillo, M.
000032199 700__ $$0(orcid)0000-0001-7294-245X$$aRezusta, A.$$uUniversidad de Zaragoza
000032199 7102_ $$12002$$2647$$aUniversidad de Zaragoza$$bDepartamento de Física Aplicada$$cOptica
000032199 7102_ $$11008$$2630$$aUniversidad de Zaragoza$$bDepartamento de Microbiología, Medicina Preventiva y Salud Pública$$cMicrobiología
000032199 773__ $$g15, 187 (2015), [8 pp.]$$pBMC Microbiol.$$tBMC MICROBIOLOGY$$x1471-2180
000032199 8564_ $$s1216129$$uhttps://zaguan.unizar.es/record/32199/files/texto_completo.pdf$$yVersión publicada
000032199 8564_ $$s106907$$uhttps://zaguan.unizar.es/record/32199/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000032199 909CO $$ooai:zaguan.unizar.es:32199$$particulos$$pdriver
000032199 951__ $$a2016-06-27-09:47:41
000032199 980__ $$aARTICLE