32217 20210121082901.0 doi 10.3390/md13095666 sideral 92243 ART-2015-92243 eng (orcid)0000-0003-1892-5063 Ceballos-Laita, L. gamma-Lindane increases microcystin synthesis in microcystis aeruginosa PCC7806 2015 Access copy available to the general public Unrestricted HCH factories, and the waste dumpsites associated to its production, have become a global environmental concern, and their runoff could pollute ground and surface waters with high levels of the pollutant. In this study, the influence of lindane (¿-HCH) on microcystin production has been investigated in Microcystis aeruginosa PCC7806. This toxic cyanobacterium is highly tolerant to ¿-lindane (20 mg/L), and produces more toxin (microcystin) in the presence of the pollutant. Microcystis degrades ¿-lindane and presence of ¿-lindane induces genes involved in its own degradation (nirA). RT-PCRsq has been used to monitor changes in levels of transcripts encoded by the mcy operon (mcyD, mcyH and mcyJ), responsible for the microcystin synthesis machinery, as well as other genes involved in its transcriptional regulation, such as ntcA and fur family members. The presence of lindane in the culture media induces mcyD expression, as well as ntcA gene transcription, while other genes, such as mcyH, (putative ABC transporter), are downregulated. The amount of microcystin found in the cells and the culture media is higher when M. aeruginosa is treated with ¿-lindane than in control cells. The results\ suggest that in a lindane polluted environment, Microcystis toxic strains may enhance their microcystin synthesis. info:eu-repo/grantAgreement/ES/MINECO/BFU2012-31458 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 3.345 2015 CHEMISTRY, MEDICINAL 13 / 59 = 0.22 2015 Q1 T1 0.775 2015 Drug Discovery 2015 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion (orcid)0000-0001-5327-4046 Calvo-Begueria, L. Lahoz, J. (orcid)0000-0002-8181-2689 Bes, M.T Universidad de Zaragoza (orcid)0000-0001-8644-4574 Fillat, M.F. Universidad de Zaragoza (orcid)0000-0002-2742-3711 Peleato, M.L Universidad de Zaragoza 1002 412 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Fisiología Vegetal 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 13, 9 (2015), 5666-5680 Marine Drugs Marine Drugs 1660-3397 610932 http://zaguan.unizar.es/record/32217/files/texto_completo.pdf Versión publicada 93928 http://zaguan.unizar.es/record/32217/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:32217 articulos driver 2021-01-21-08:16:09 ARTICLE