doi:10.3390/molecules19010672engAntonini, L. V.Peregrina, J. R.Angulo, J.Medina Trullenque, María MilagrosNieto, P. M.A STD-NMR study of the interaction of the Anabaena Ferredoxin-NAD P+ reductase with the CoenzymeART-2014-85181Ferredoxin-NADP+ reductase (FNR) catalyzes the electron transfer from ferredoxin to NADP+ via its flavin FAD cofactor. To get further insights in the architecture of the transient complexes produced during the hydride transfer event between the enzyme and the NADP+ coenzyme we have applied NMR spectroscopy using Saturation Transfer Difference (STD) techniques to analyze the interaction between FNRox and the oxidized state of its NADP+ coenzyme. We have found that STD NMR, together with the use of selected mutations on FNR and of the non-FNR reacting coenzyme analogue NAD+, are appropriate tools to provide further information about the the interaction epitope.2014http://zaguan.unizar.es/record/3246010.3390/molecules19010672http://zaguan.unizar.es/record/32460oai:zaguan.unizar.es:32460info:eu-repo/grantAgreement/ES/MICINN/BIO2010-14983info:eu-repo/grantAgreement/ES/MICINN/CTQ2012-32605MOLECULES 19, 1 (2014), 672-685byhttp://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccess