000032490 001__ 32490 000032490 005__ 20220208112843.0 000032490 0247_ $$2doi$$a10.1371/journal.pone.0097768 000032490 0248_ $$2sideral$$a92398 000032490 037__ $$aART-2014-92398 000032490 041__ $$aeng 000032490 100__ $$0(orcid)0000-0001-7098-2327$$aGarza, M.C. 000032490 245__ $$aDistribution of peripheral PrPScin sheep with naturally acquired scrapie 000032490 260__ $$c2014 000032490 5060_ $$aAccess copy available to the general public$$fUnrestricted 000032490 5203_ $$aAccumulation of prion protein (PrPSc) in the central nervous system is the hallmark of transmissible spongiform encephalopathies. However, in some of these diseases such as scrapie or chronic wasting disease, the PrPSc can also accumulate in other tissues, particularly in the lymphoreticular system. In recent years, PrPSc in organs other than nervous and lymphoid have been described, suggesting that distribution of this protein in affected individuals may be much larger than previously thought. In the present study, 11 non-nervous/non-lymphoid organs from 16 naturally scrapie infected sheep in advanced stages of the disease were examined for the presence of PrPSc. Fourteen infected sheep were of the ARQ/ARQ PRNP genotype and 2 of the VRQ/VRQ, where the letters A, R, Q, and V represent the codes for amino-acids alanine, arginine, glutamine and valine, respectively. Adrenal gland, pancreas, heart, skin, urinary bladder and mammary gland were positive for PrPSc by immunohistochemistry and IDEXX HerdChek scrapie/BSE Antigen EIA Test in at least one animal. Lung, liver, kidney and skeletal muscle exhibited PrPSc deposits by immunohistochemistry only. To our knowledge, this is the first report regarding the presence of PrPSc in the heart, pancreas and urinary bladder in naturally acquired scrapie infections. In some other organs examined, in which PrPSc had been previously detected, PrPSc immunolabeling was observed to be associated with new structures within those organs. The results of the present study illustrate a wide dissemination of PrPSc in both ARQ/ARQ and VRQ/VRQ infected sheep, even when the involvement of the lymphoreticular system is scarce or absent, thus highlighting the role of the peripheral nervous system in the spread of PrPSc. 000032490 536__ $$9info:eu-repo/grantAgreement/ES/MEC/AP2007-03842$$9info:eu-repo/grantAgreement/ES/DGA/218-315-3$$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/EFA205-11 000032490 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000032490 590__ $$a3.234$$b2014 000032490 591__ $$aMULTIDISCIPLINARY SCIENCES$$b9 / 57 = 0.158$$c2014$$dQ1$$eT1 000032490 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000032490 700__ $$0(orcid)0000-0002-2787-9671$$aMonzón, M.$$uUniversidad de Zaragoza 000032490 700__ $$0(orcid)0000-0002-1590-3347$$aMarín, B.$$uUniversidad de Zaragoza 000032490 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, J.J.$$uUniversidad de Zaragoza 000032490 700__ $$0(orcid)0000-0002-7453-1766$$aMonleon, E.$$uUniversidad de Zaragoza 000032490 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000032490 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología 000032490 773__ $$g9, 5 (2014), e97768 [14 pp.]$$pPLoS One$$tPLoS ONE$$x1932-6203 000032490 8564_ $$s2660555$$uhttps://zaguan.unizar.es/record/32490/files/texto_completo.pdf$$yVersión publicada 000032490 8564_ $$s123530$$uhttps://zaguan.unizar.es/record/32490/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000032490 909CO $$ooai:zaguan.unizar.es:32490$$particulos$$pdriver 000032490 951__ $$a2022-02-08-11:23:02 000032490 980__ $$aARTICLE