000032492 001__ 32492
000032492 005__ 20191105115756.0
000032492 0247_ $$2doi$$a10.1093/nar/gku123
000032492 0248_ $$2sideral$$a86702
000032492 037__ $$aART-2014-86702
000032492 041__ $$aeng
000032492 100__ $$aGonzález, Andrés
000032492 245__ $$aThe FurA regulon in Anabaena sp. PCC 7120: In silico prediction and experimental validation of novel target genes
000032492 260__ $$c2014
000032492 5060_ $$aAccess copy available to the general public$$fUnrestricted
000032492 5203_ $$aIn the filamentous cyanobacterium Anabaena sp. PCC 7120, the ferric uptake regulator FurA functions as a global transcriptional regulator. Despite several analyses have focused on elucidating the FurA-regulatory network, the number of target genes described for this essential transcription factor is limited to a handful of examples. In this article, we combine an in silico genome-wide predictive approach with experimental determinations to better define the FurA regulon. Predicted FurA-binding sites were identified upstream of 215 genes belonging to diverse functional categories including iron homeostasis, photosynthesis and respiration, heterocyst differentiation, oxidative stress defence and light-dependent signal transduction mechanisms, among others. The probabilistic model proved to be effective at discerning FurA boxes from non-cognate sequences, while subsequent electrophoretic mobility shift assay experiments confirmed the in vitro specific binding of FurA to at least 20 selected predicted targets. Gene-expression analyses further supported the dual role of FurA as transcriptional modulator that can act both as repressor and as activator. In either role, the in vitro affinity of the protein to its target sequences is strongly dependent on metal co-regulator and reducing conditions, suggesting that FurA couples in vivo iron homeostasis and the response to oxidative stress to major physiological processes in cyanobacteria.
000032492 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/BFU2010-16297$$9info:eu-repo/grantAgreement/ES/MINECO/BFU2012-31458
000032492 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000032492 590__ $$a9.112$$b2014
000032492 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b20 / 290 = 0.069$$c2014$$dQ1$$eT1
000032492 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000032492 700__ $$0(orcid)0000-0002-3268-8730$$aEspinosa Angarica, Vladimir$$uUniversidad de Zaragoza
000032492 700__ $$aSancho, Javier
000032492 700__ $$0(orcid)0000-0001-8644-4574$$aFillat, María F.$$uUniversidad de Zaragoza
000032492 7102_ $$11002$$2X$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cProy. investigación DEA
000032492 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000032492 773__ $$g42, 8 (2014), 4833-4846$$pNucleic acids res.$$tNucleic Acids Research$$x0305-1048
000032492 8564_ $$s1233104$$uhttps://zaguan.unizar.es/record/32492/files/texto_completo.pdf
000032492 8564_ $$s132754$$uhttps://zaguan.unizar.es/record/32492/files/texto_completo.jpg?subformat=icon$$xicon
000032492 909CO $$ooai:zaguan.unizar.es:32492$$particulos$$pdriver
000032492 951__ $$a2019-11-05-11:49:14
000032492 980__ $$aARTICLE