000044963 001__ 44963
000044963 005__ 20191105115757.0
000044963 0247_ $$2doi$$a10.1107/S1399004715002485
000044963 0248_ $$2sideral$$a90011
000044963 037__ $$aART-2015-90011
000044963 041__ $$aeng
000044963 100__ $$aAlonso-García, N.
000044963 245__ $$aCombination of X-ray crystallography, SAXS and DEER to obtain the structure of the FnIII-3,4 domains of integrin a6b4
000044963 260__ $$c2015
000044963 5060_ $$aAccess copy available to the general public$$fUnrestricted
000044963 5203_ $$aIntegrin alpha6beta4 is a major component of hemidesmosomes that mediate the stable anchorage of epithelial cells to the underlying basement membrane. Integrin alpha6beta4 has also been implicated in cell proliferation and migration and in carcinoma progression. The third and fourth fibronectin type III domains (FnIII-3,4) of integrin beta4 mediate binding to the hemidesmosomal proteins BPAG1e and BPAG2, and participate in signalling. Here, it is demonstrated that X-ray crystallography, small-angle X-ray scattering and double electron– electron resonance (DEER) complement each other to solve the structure of the FnIII-3,4 region. The crystal structures of the individual FnIII-3 and FnIII-4 domains were solved and the relative arrangement of the FnIII domains was elucidated by combining DEER with site-directed spin labelling. Multiple structures of the interdomain linker were modelled by Monte Carlo methods complying with DEER constraints, and the final structures were selected against experimental scattering data. FnIII-3,4 has a compact and cambered flat structure with an evolutionary conserved surface that is likely to correspond to a protein-interaction site. Finally, this hybrid method is of general application for the study of other macromolecules and complexes.
000044963 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/BFU2009-08389$$9info:eu-repo/grantAgreement/ES/MINECO/BFU2012-32847
000044963 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000044963 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000044963 700__ $$0(orcid)0000-0002-1827-1250$$aGarcía-Rubio, I.
000044963 700__ $$aManso, J.A.
000044963 700__ $$aBuey, R.M.
000044963 700__ $$aUrien, H.
000044963 700__ $$aSonnenberg, A.
000044963 700__ $$aJeschke, G.
000044963 700__ $$ade Pereda, J.M.
000044963 773__ $$gD71 (2015), 969-985$$pActa crystallogr., D Biol. crystallogr.$$tACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY$$x0907-4449
000044963 8564_ $$s3294398$$uhttps://zaguan.unizar.es/record/44963/files/texto_completo.pdf$$yVersión publicada
000044963 8564_ $$s98592$$uhttps://zaguan.unizar.es/record/44963/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000044963 909CO $$ooai:zaguan.unizar.es:44963$$particulos$$pdriver
000044963 951__ $$a2019-11-05-11:49:14
000044963 980__ $$aARTICLE