Role of Mechanical Cues in Cell Differentiation and Proliferation: A 3D Numerical Model
Resumen: Cell differentiation, proliferation and migration are essential processes in tissue regenera- tion. Experimental evidence confirms that cell differentiation or proliferation can be regulat- ed according to the extracellular matrix stiffness. For instance, mesenchymal stem cells (MSCs) can differentiate to neuroblast, chondrocyte or osteoblast within matrices mimicking the stiffness of their native substrate. However, the precise mechanisms by which the sub- strate stiffness governs cell differentiation or proliferation are not well known. Therefore, a mechano-sensing computational model is here developed to elucidate how substrate stiff- ness regulates cell differentiation and/or proliferation during cell migration. In agreement with experimental observations, it is assumed that internal deformation of the cell (a me- chanical signal) together with the cell maturation state directly coordinates cell differentia- tion and/or proliferation. Our findings indicate that MSC differentiation to neurogenic, chondrogenic or osteogenic lineage specifications occurs within soft (0.1-1 kPa), intermedi- ate (20-25 kPa) or hard (30-45 kPa) substrates, respectively. These results are consistent with well-known experimental observations. Remarkably, when a MSC differentiate to a compatible phenotype, the average net traction force depends on the substrate stiffness in such a way that it might increase in intermediate and hard substrates but it would reduce in a soft matrix. However, in all cases the average net traction force considerably increases at the instant of cell proliferation because of cell-cell interaction. Moreover cell differentiation and proliferation accelerate with increasing substrate stiffness due to the decrease in the cell maturation time. Thus, the model provides insights to explain the hypothesis that sub- strate stiffness plays a key role in regulating cell fate during mechanotaxis.
Idioma: Inglés
DOI: 10.1371/journal.pone.0124529
Año: 2015
Publicado en: PLoS ONE 10, 5 (2015), e0124529 [23 pp
ISSN: 1932-6203

Factor impacto JCR: 3.057 (2015)
Categ. JCR: MULTIDISCIPLINARY SCIENCES rank: 11 / 62 = 0.177 (2015) - Q1 - T1
Factor impacto SCIMAGO:

Financiación: info:eu-repo/grantAgreement/ES/MINECO/MAT2013-46467-C4-3-R
Tipo y forma: Article (Published version)
Área (Departamento): Área Mec.Med.Cont. y Teor.Est. (Dpto. Ingeniería Mecánica)

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