000046912 001__ 46912
000046912 005__ 20210820090341.0
000046912 0247_ $$2doi$$a10.1371/journal.pone.0127722
000046912 0248_ $$2sideral$$a91023
000046912 037__ $$aART-2015-91023
000046912 041__ $$aeng
000046912 100__ $$aRibeiro, F.O.
000046912 245__ $$aIn silico mechano-chemical model of bone healing for the regeneration of critical defects: The effect of BMP-2
000046912 260__ $$c2015
000046912 5060_ $$aAccess copy available to the general public$$fUnrestricted
000046912 5203_ $$aThe healing of bone defects is a challenge for both tissue engineering and modern ortho- paedics. This problem has been addressed through the study of scaffold constructs com- bined with mechanoregulatory theories, disregarding the influence of chemical factors and their respective delivery devices. Of the chemical factors involved in the bone healing pro- cess, bone morphogenetic protein-2 (BMP-2) has been identified as one of the most power- ful osteoinductive proteins. The aim of this work is to develop and validate a mechano- chemical regulatory model to study the effect of BMP-2 on the healing of large bone defects in silico. We first collected a range of quantitative experimental data from the literature con- cerning the effects of BMP-2 on cellular activity, specifically proliferation, migration, differen- tiation, maturation and extracellular matrix production. These data were then used to define a model governed by mechano-chemical stimuli to simulate the healing of large bone de- fects under the following conditions: natural healing, an empty hydrogel implanted in the de- fect and a hydrogel soaked with BMP-2 implanted in the defect. For the latter condition, successful defect healing was predicted, in agreement with previous in vivo experiments. Further in vivo comparisons showed the potential of the model, which accurately predicted bone tissue formation during healing, bone tissue distribution across the defect and the quantity of bone inside the defect. The proposed mechano-chemical model also estimated the effect of BMP-2 on cells and the evolution of healing in large bone defects. This novel in silico tool provides valuable insight for bone tissue regeneration strategies.
000046912 536__ $$9info:eu-repo/grantAgreement/EUR/FP7/ERC2012-StG-306751$$9info:eu-repo/grantAgreement/ES/MINECO/DPI2012-32888
000046912 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000046912 590__ $$a3.057$$b2015
000046912 591__ $$aMULTIDISCIPLINARY SCIENCES$$b11 / 62 = 0.177$$c2015$$dQ1$$eT1
000046912 592__ $$a1.427$$b2015
000046912 593__ $$aAgricultural and Biological Sciences (miscellaneous)$$c2015$$dQ1
000046912 593__ $$aMedicine (miscellaneous)$$c2015$$dQ1
000046912 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2015$$dQ1
000046912 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000046912 700__ $$0(orcid)0000-0002-1878-8997$$aGómez-Benito, M.J.$$uUniversidad de Zaragoza
000046912 700__ $$aFolgado, J.
000046912 700__ $$aFernandes, P.R.
000046912 700__ $$0(orcid)0000-0002-9864-7683$$aGarcía-Aznar, J.M.$$uUniversidad de Zaragoza
000046912 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000046912 773__ $$g10, 6 (2015), 0127722 [25 p]$$pPLoS One$$tPloS one$$x1932-6203
000046912 8564_ $$s3039256$$uhttps://zaguan.unizar.es/record/46912/files/texto_completo.pdf$$yVersión publicada
000046912 8564_ $$s103263$$uhttps://zaguan.unizar.es/record/46912/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000046912 909CO $$ooai:zaguan.unizar.es:46912$$particulos$$pdriver
000046912 951__ $$a2021-08-20-08:35:30
000046912 980__ $$aARTICLE