000047389 001__ 47389 000047389 005__ 20200221144332.0 000047389 0247_ $$2doi$$a10.1186/s13567-015-0295-8 000047389 0248_ $$2sideral$$a93425 000047389 037__ $$aART-2016-93425 000047389 041__ $$aeng 000047389 100__ $$0(orcid)0000-0002-0827-110X$$aHedman, C. 000047389 245__ $$aTransmission of sheep-bovine spongiform encephalopathy to pigs 000047389 260__ $$c2016 000047389 5060_ $$aAccess copy available to the general public$$fUnrestricted 000047389 5203_ $$aExperimental transmission of the bovine spongiform encephalopathy (BSE) agent has been successfully reported in pigs inoculated via three simultaneous distinct routes (intracerebral, intraperitoneal and intravenous). Sheep derived BSE (Sh-BSE) is transmitted more efficiently than the original cattle-BSE isolate in a transgenic mouse model expressing porcine prion protein. However, the neuropathology and distribution of Sh-BSE in pigs as natural hosts, and susceptibility to this agent, is unknown. In the present study, seven pigs were intracerebrally inoculated with Sh-BSE prions. One pig was euthanized for analysis in the preclinical disease stage. The remaining six pigs developed neurological signs and histopathology revealed severe spongiform changes accompanied by astrogliosis and microgliosis throughout the central nervous system. Intracellular and neuropil-associated pathological prion protein (PrPSc) deposition was consistently observed in different brain sections and corroborated by Western blot. PrPSc was detected by immunohistochemistry and enzyme immunoassay in the following tissues in at least one animal: lymphoid tissues, peripheral nerves, gastrointestinal tract, skeletal muscle, adrenal gland and pancreas. PrPSc deposition was revealed by immunohistochemistry alone in the retina, optic nerve and kidney. These results demonstrate the efficient transmission of Sh-BSE in pigs and show for the first time that in this species propagation of bovine PrPSc in a wide range of peripheral tissues is possible. These results provide important insight into the distribution and detection of prions in non-ruminant animals. 000047389 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000047389 590__ $$a2.798$$b2016 000047389 591__ $$aVETERINARY SCIENCES$$b5 / 136 = 0.037$$c2016$$dQ1$$eT1 000047389 592__ $$a1.44$$b2016 000047389 593__ $$aVeterinary (miscellaneous)$$c2016$$dQ1 000047389 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000047389 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, R.$$uUniversidad de Zaragoza 000047389 700__ $$aMarín, B. 000047389 700__ $$aCobrière, F. 000047389 700__ $$0(orcid)0000-0003-3352-740X$$aFilali, H. 000047389 700__ $$0(orcid)0000-0002-8712-2275$$aVazquez, F.$$uUniversidad de Zaragoza 000047389 700__ $$0(orcid)0000-0003-3016-9125$$aPitarch, J.L.$$uUniversidad de Zaragoza 000047389 700__ $$0(orcid)0000-0002-6158-691X$$aVargas, A.$$uUniversidad de Zaragoza 000047389 700__ $$0(orcid)0000-0001-5105-6133$$aAcín, C.$$uUniversidad de Zaragoza 000047389 700__ $$0(orcid)0000-0001-6629-6842$$aMoreno, B.$$uUniversidad de Zaragoza 000047389 700__ $$0(orcid)0000-0002-0935-7941$$aPumarola, M. 000047389 700__ $$aAndreoletti, O. 000047389 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, J.J.$$uUniversidad de Zaragoza 000047389 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000047389 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal 000047389 773__ $$g47, 14 (2016), [15 pp.]$$pVet. res.$$tVeterinary Research$$x0928-4249 000047389 8564_ $$s3380169$$uhttps://zaguan.unizar.es/record/47389/files/texto_completo.pdf$$yVersión publicada 000047389 8564_ $$s103946$$uhttps://zaguan.unizar.es/record/47389/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000047389 909CO $$ooai:zaguan.unizar.es:47389$$particulos$$pdriver 000047389 951__ $$a2020-02-21-13:45:34 000047389 980__ $$aARTICLE