47389 20200221144332.0 doi 10.1186/s13567-015-0295-8 sideral 93425 ART-2016-93425 eng (orcid)0000-0002-0827-110X Hedman, C. Transmission of sheep-bovine spongiform encephalopathy to pigs 2016 Access copy available to the general public Unrestricted Experimental transmission of the bovine spongiform encephalopathy (BSE) agent has been successfully reported in pigs inoculated via three simultaneous distinct routes (intracerebral, intraperitoneal and intravenous). Sheep derived BSE (Sh-BSE) is transmitted more efficiently than the original cattle-BSE isolate in a transgenic mouse model expressing porcine prion protein. However, the neuropathology and distribution of Sh-BSE in pigs as natural hosts, and susceptibility to this agent, is unknown. In the present study, seven pigs were intracerebrally inoculated with Sh-BSE prions. One pig was euthanized for analysis in the preclinical disease stage. The remaining six pigs developed neurological signs and histopathology revealed severe spongiform changes accompanied by astrogliosis and microgliosis throughout the central nervous system. Intracellular and neuropil-associated pathological prion protein (PrPSc) deposition was consistently observed in different brain sections and corroborated by Western blot. PrPSc was detected by immunohistochemistry and enzyme immunoassay in the following tissues in at least one animal: lymphoid tissues, peripheral nerves, gastrointestinal tract, skeletal muscle, adrenal gland and pancreas. PrPSc deposition was revealed by immunohistochemistry alone in the retina, optic nerve and kidney. These results demonstrate the efficient transmission of Sh-BSE in pigs and show for the first time that in this species propagation of bovine PrPSc in a wide range of peripheral tissues is possible. These results provide important insight into the distribution and detection of prions in non-ruminant animals. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 2.798 2016 VETERINARY SCIENCES 5 / 136 = 0.037 2016 Q1 T1 1.44 2016 Veterinary (miscellaneous) 2016 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion (orcid)0000-0002-2746-3932 Bolea, R. Universidad de Zaragoza Marín, B. Cobrière, F. (orcid)0000-0003-3352-740X Filali, H. (orcid)0000-0002-8712-2275 Vazquez, F. Universidad de Zaragoza (orcid)0000-0003-3016-9125 Pitarch, J.L. Universidad de Zaragoza (orcid)0000-0002-6158-691X Vargas, A. Universidad de Zaragoza (orcid)0000-0001-5105-6133 Acín, C. Universidad de Zaragoza (orcid)0000-0001-6629-6842 Moreno, B. Universidad de Zaragoza (orcid)0000-0002-0935-7941 Pumarola, M. Andreoletti, O. (orcid)0000-0002-7173-7216 Badiola, J.J. Universidad de Zaragoza 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 47, 14 (2016), [15 pp.] Vet. res. Veterinary Research 0928-4249 3380169 http://zaguan.unizar.es/record/47389/files/texto_completo.pdf Versión publicada 103946 http://zaguan.unizar.es/record/47389/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:47389 articulos driver 2020-02-21-13:45:34 ARTICLE