47854 20231023123340.0 doi 10.1167/iovs.15-17257 sideral 92496 ART-2015-92496 eng Segura, Francisco Universidad de Zaragoza (orcid)0000-0002-9250-9060 Assessment of Visual and Chromatic Functions in a Rodent Model of Retinal Degeneration 2015 Access copy available to the general public Unrestricted Purpose: We evaluated the photoreceptor response of pigmented P23H and normal pigmented Long Evans (LE) rats over time using functional tests in variable lighting conditions. Methods: Pigmented P23H rats were studied by optomotor testing and electroretinogram (ERG) recordings at P30, P150, and P240. Pigmented LE rats were used as a normal wild-type control. Stimuli were modified with colored filters. Neutral density filters were used to reduce luminance. Results: Age-related decreases in visual acuity (VA) and contrast sensitivity (CS) were observed in P23H rats. Good correlations in measurements without filter and with green filter were observed between LE and P23H P30 rat values. Differences between groups were smaller with red and purple filters. A strong relationship with luminance was observed in LE rats (VA and CS) and with P23H P30 rats (CS). A decline in the ERG responses of P23H rats was consistent with the gradual loss of photoreceptors. Differences in a- and b-wave amplitudes with different colored filters were negligible with the exception of the red filter, which resulted in smaller responses. Conclusions: Visual function parameters decreased with age in pigmented P23H rats. Irrespective of luminance, color filter, and retinal degeneration, minimum thresholds of VA and CS were found. Smaller differences than expected were found using color filters. Responses to functional tests at long wavelengths were observed, where there is very low photoreceptor spectral sensitivity. The use of filters with functional testing could minimize light-induced retinal damage in rats. info:eu-repo/grantAgreement/ES/DGA/B99 info:eu-repo/grantAgreement/ES/ISCIII/PI13-01124 info:eu-repo/grantAgreement/ES/ISCIII/PS090-1854 info:eu-repo/grantAgreement/ES/ISCIII/RETICS-RD12-0034-0010 info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 3.427 2015 OPHTHALMOLOGY 6 / 56 = 0.107 2015 Q1 T1 2.011 2015 Ophthalmology 2015 Q1 Sensory Systems 2015 Q1 Cellular and Molecular Neuroscience 2015 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Sanchez-Cano, Ana Universidad de Zaragoza (orcid)0000-0002-5621-1937 Jarabo Lallana, Sebastián Universidad de Zaragoza (orcid)0000-0002-2523-8598 Lopez de la Fuente, Carmen Universidad de Zaragoza (orcid)0000-0002-7471-5362 Cuenca, Nicolas Villegas-Perez, Maria P Pinilla, Isabel Universidad de Zaragoza (orcid)0000-0003-0349-9997 1004 646 Universidad de Zaragoza Dpto. Cirugía,Ginecol.Obstetr. Área Oftalmología 2002 647 Universidad de Zaragoza Dpto. Física Aplicada Área Óptica 56, 11 (2015), 6275-6283 Investig. ophthalmol. vis. sci. Investigative ophthalmology & visual science 0146-0404 1120703 http://zaguan.unizar.es/record/47854/files/texto_completo.pdf Postprint 122724 http://zaguan.unizar.es/record/47854/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:47854 articulos driver 2023-10-23-12:20:32 ARTICLE