48112 20231006143251.0 doi 10.1099/jgv.0.000292 sideral 93736 ART-2015-93736 eng Mediano, Diego R. (orcid)0000-0002-8692-1382 Characterization of mesenchymal stem cells in sheep naturally infected with scrapie 2015 Access copy available to the general public Unrestricted Mesenchymal stem cells (MSCs) can be infected with prions and have been proposed as in vitro cell-based models for prion replication. In addition, autologous MSCs are of interest for cell therapy in neurodegenerative diseases. To the best of our knowledge, the effect of prion diseases on the characteristics of these cells has never been investigated. Here, we analysed the properties of MSCs obtained from bone marrow (BM-MSCs) and peripheral blood (PB-MSCs) of sheep naturally infected with scrapie — a large mammal model for the study of prion diseases. After three passages of expansion, MSCs derived from scrapie animals displayed similar adipogenic, chondrogenic and osteogenic differentiation ability as cells from healthy controls, although a subtle decrease in the proliferation potential was observed. Exceptionally, mesenchymal markers such as CD29 were significantly upregulated at the transcript level compared with controls. Scrapie MSCs were able to transdifferentiate into neuron-like cells, but displayed lower levels of neurogenic markers at basal conditions, which could limit this potential. The expression levels of cellular prion protein (PrPC) were highly variable between cultures, and no significant differences were observed between control and scrapie-derived MSCs. However, during neurogenic differentiation the expression of PrPC was upregulated in MSCs. This characteristic could be useful for developing in vitro models for prion replication. Despite the infectivity reported for MSCs obtained from scrapie-infected mice and Creutzfeldt–Jakob disease patients, protein misfolding cyclic amplification did not detect PrPSc in BM- or PB-MSCs from scrapie-infected sheep, which limits their use for in vivo diagnosis for scrapie. info:eu-repo/grantAgreement/ES/DGA/CTPP2-13 info:eu-repo/grantAgreement/ES/DGA/CTP2013-P05 info:eu-repo/grantAgreement/ES/UZ/2012-BIO-03 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 3.192 2015 VIROLOGY 13 / 33 = 0.394 2015 Q2 T2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY 46 / 161 = 0.286 2015 Q2 T1 1.738 2015 Virology 2015 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Sanz-Rubio, David (orcid)0000-0002-5228-248X Bolea, Rosa Universidad de Zaragoza (orcid)0000-0002-2746-3932 Marín, Belén Universidad de Zaragoza (orcid)0000-0002-1590-3347 Vázquez, Francisco J. Universidad de Zaragoza (orcid)0000-0002-8712-2275 Remacha, Ana R. Universidad de Zaragoza (orcid)0000-0002-1075-8267 López-Pérez, Óscar Universidad de Zaragoza (orcid)0000-0003-2454-2114 Fernández-Borges, Natalia Castilla, Joaquín Zaragoza, Pilar Universidad de Zaragoza (orcid)0000-0001-5740-0185 Badiola, Juan J. Universidad de Zaragoza (orcid)0000-0002-7173-7216 Rodellar, Clementina Universidad de Zaragoza (orcid)0000-0003-3289-2675 Martín-Burriel, Inmaculada Universidad de Zaragoza (orcid)0000-0001-6016-4726 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1009 X Universidad de Zaragoza Dpto. Patología Animal Contratos Servicios HRA 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 96 (2015), 3715-3726 J. gen. virol. JOURNAL OF GENERAL VIROLOGY 0022-1317 6401384 http://zaguan.unizar.es/record/48112/files/texto_completo.pdf Versión publicada 106716 http://zaguan.unizar.es/record/48112/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:48112 articulos driver 2023-10-06-14:05:54 ARTICLE