48158 20170504095947.0 doi 10.1038/srep04913 sideral 86874 ART-2014-86874 eng (orcid)0000-0003-2685-5739 Nerin, C. Universidad de Zaragoza Compounds from multilayer plastic bags cause reproductive failures in artificial insemination 2014 Access copy available to the general public Unrestricted High levels of reproductive failure were detected in some Spanish sow farms in the Spring of 2010. Regular returns to estrus and variable reductions in litter size were observed. The problem started suddenly and did not appear to be related to the quality of the ejaculates, disease, alterations of body condition or any other apparent reasons. Subsequent studies determined that the problem was the origin of the plastic bags used for semen storage. Chemical analysis of the suspicious bags identified unexpected compounds such as BADGE, a cyclic lactone and an unknown phthalate that leached into the semen at concentrations of 0.2 to 2.5 mg/L. Spermatozoa preserved in these bags passed all of the routine quality control tests, and no differences were observed between storage in the control and suspicious bags (p . 0.05). In vitro fecundation tests and endocrine profiler panel analysis (EPP) did not show any alterations, whereas the in vivo tests confirmed the described failure. This is the first described relationship between reproductive failure and toxic compounds released from plastic bags. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 5.578 2014 MULTIDISCIPLINARY SCIENCES 5 / 57 = 0.088 2014 Q1 T1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Ubeda, J.L. (orcid)0000-0002-3703-7902 Alfaro, P. Dahmani, Y. (orcid)0000-0002-0759-9170 Aznar, M. Universidad de Zaragoza (orcid)0000-0003-2638-9221 Canellas, E. Ausejo, R. 2009 750 Universidad de Zaragoza Departamento de Química Analítica Química Analítica 4 (2014), 4913 [10 pp.] Sci. rep. SCIENTIFIC REPORTS 2045-2322 614405 http://zaguan.unizar.es/record/48158/files/texto_completo.pdf Versión publicada 101526 http://zaguan.unizar.es/record/48158/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:48158 articulos driver 2017-05-04-09:53:09 ARTICLE