000048334 001__ 48334
000048334 005__ 20210121114511.0
000048334 0247_ $$2doi$$a10.1016/j.eimc.2015.01.015
000048334 0248_ $$2sideral$$a92391
000048334 037__ $$aART-2015-92391
000048334 041__ $$aeng
000048334 100__ $$aGonzález-Domínguez, M
000048334 245__ $$aAntimicrobial resistance, virulence factorsand genetic lineages of hospital-onsetmethicillin-resistant Staphylococcus aureus isolates detected in a hospital in Zaragoza
000048334 260__ $$c2015
000048334 5060_ $$aAccess copy available to the general public$$fUnrestricted
000048334 5203_ $$aIntroduction
MRSA population dynamics is undergoing significant changes, and for this reason it is important to know which clones are circulating in our nosocomial environment.
Materials and methods
A total of 118 MRSA isolates were collected from clinical samples from patients with previous hospital or healthcare contact (named as hospital-onset MRSA (HO-MRSA)) during a one year period. Susceptibility testing was performed by disk diffusion and microdilution. The presence of resistance genes and virulence factors were tested by PCR. All isolates were typed by SCCmec, spa and agr typing. PFGE and MLST were applied to a selection of them.
Results
Eighty-three HO-MRSA isolates (70.3%) were resistant to any antibiotic included in the macrolide–lincosamide–streptogramin B group. Among these isolates, the M phenotype was the most frequent (73.5%). One hundred and seven of HO-MRSA isolates (90.7%) showed aminoglycoside resistance. The combination aac(6')-Ie-aph(2")-Ia + ant(4')-Ia genes was the most frequent (22.4%). Tetracycline resistance rates in HO-MRSA isolates were low (3.4%), although a high level of mupirocin resistance was observed (25.4%). Most of the HO-MRSA isolates (approximately 90%) showed SCCmec type IVc and agr type II. Fifteen unrelated pulsotypes were identified. CC5 was the most prevalent (88.1%), followed by CC8 (5.9%), CC22 (2.5%), CC398 (2.5%) and CC1 (0.8%).
Conclusion
CC5/ST125/t067 lineage was the most frequent. This lineage was related to aminoglycoside resistance, and to a lesser extent, with macrolide resistance. The presence of international clones as EMRSA-15 (CC22/ST22), European clones as CC5/ST228, community clones related to CC1 or CC8 and livestock associated clones, as CC398, were observed in a low percentage.
000048334 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B24-211130
000048334 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000048334 590__ $$a1.53$$b2015
000048334 591__ $$aINFECTIOUS DISEASES$$b59 / 83 = 0.711$$c2015$$dQ3$$eT3
000048334 591__ $$aMICROBIOLOGY$$b97 / 123 = 0.789$$c2015$$dQ4$$eT3
000048334 592__ $$a0.378$$b2015
000048334 593__ $$aMicrobiology (medical)$$c2015$$dQ3
000048334 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000048334 700__ $$0(orcid)0000-0002-9742-1463$$aSeral, C$$uUniversidad de Zaragoza
000048334 700__ $$aPotel, C
000048334 700__ $$aConstenla, L
000048334 700__ $$aAlgarate, S
000048334 700__ $$aGude, MJ
000048334 700__ $$aAlvarez, M
000048334 700__ $$0(orcid)0000-0002-2519-701X$$aCastillo FJ$$uUniversidad de Zaragoza
000048334 7102_ $$11008$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Microbiología
000048334 773__ $$g33, 9 (2015), 590-596$$pEnferm. infecc. microbiol. clín.$$tEnfermedades Infecciosas y Microbiologia Clinica$$x0213-005X
000048334 8564_ $$s271681$$uhttps://zaguan.unizar.es/record/48334/files/texto_completo.pdf$$yPostprint
000048334 8564_ $$s21827$$uhttps://zaguan.unizar.es/record/48334/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
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000048334 951__ $$a2021-01-21-10:57:33
000048334 980__ $$aARTICLE