48334 20210121114511.0 doi 10.1016/j.eimc.2015.01.015 sideral 92391 ART-2015-92391 eng González-Domínguez, M Antimicrobial resistance, virulence factorsand genetic lineages of hospital-onsetmethicillin-resistant Staphylococcus aureus isolates detected in a hospital in Zaragoza 2015 Access copy available to the general public Unrestricted Introduction MRSA population dynamics is undergoing significant changes, and for this reason it is important to know which clones are circulating in our nosocomial environment. Materials and methods A total of 118 MRSA isolates were collected from clinical samples from patients with previous hospital or healthcare contact (named as hospital-onset MRSA (HO-MRSA)) during a one year period. Susceptibility testing was performed by disk diffusion and microdilution. The presence of resistance genes and virulence factors were tested by PCR. All isolates were typed by SCCmec, spa and agr typing. PFGE and MLST were applied to a selection of them. Results Eighty-three HO-MRSA isolates (70.3%) were resistant to any antibiotic included in the macrolide–lincosamide–streptogramin B group. Among these isolates, the M phenotype was the most frequent (73.5%). One hundred and seven of HO-MRSA isolates (90.7%) showed aminoglycoside resistance. The combination aac(6')-Ie-aph(2")-Ia + ant(4')-Ia genes was the most frequent (22.4%). Tetracycline resistance rates in HO-MRSA isolates were low (3.4%), although a high level of mupirocin resistance was observed (25.4%). Most of the HO-MRSA isolates (approximately 90%) showed SCCmec type IVc and agr type II. Fifteen unrelated pulsotypes were identified. CC5 was the most prevalent (88.1%), followed by CC8 (5.9%), CC22 (2.5%), CC398 (2.5%) and CC1 (0.8%). Conclusion CC5/ST125/t067 lineage was the most frequent. This lineage was related to aminoglycoside resistance, and to a lesser extent, with macrolide resistance. The presence of international clones as EMRSA-15 (CC22/ST22), European clones as CC5/ST228, community clones related to CC1 or CC8 and livestock associated clones, as CC398, were observed in a low percentage. info:eu-repo/grantAgreement/ES/DGA/B24-211130 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 1.53 2015 INFECTIOUS DISEASES 59 / 83 = 0.711 2015 Q3 T3 MICROBIOLOGY 97 / 123 = 0.789 2015 Q4 T3 0.378 2015 Microbiology (medical) 2015 Q3 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion (orcid)0000-0002-9742-1463 Seral, C Universidad de Zaragoza Potel, C Constenla, L Algarate, S Gude, MJ Alvarez, M (orcid)0000-0002-2519-701X Castillo FJ Universidad de Zaragoza 1008 630 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Microbiología 33, 9 (2015), 590-596 Enferm. infecc. microbiol. clín. Enfermedades Infecciosas y Microbiologia Clinica 0213-005X 271681 http://zaguan.unizar.es/record/48334/files/texto_completo.pdf Postprint 21827 http://zaguan.unizar.es/record/48334/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:48334 articulos driver 2021-01-21-10:57:33 ARTICLE