000048374 001__ 48374
000048374 005__ 20200221144348.0
000048374 0247_ $$2doi$$a10.1371/journal.pone.0148811
000048374 0248_ $$2sideral$$a93897
000048374 037__ $$aART-2016-93897
000048374 041__ $$aeng
000048374 100__ $$0(orcid)0000-0002-0308-1390$$aRamo, Ana$$uUniversidad de Zaragoza
000048374 245__ $$aIntra-Species Diversity and Panmictic Structure of Cryptosporidium parvum Populations in Cattle Farms in Northern Spain
000048374 260__ $$c2016
000048374 5060_ $$aAccess copy available to the general public$$fUnrestricted
000048374 5203_ $$aThe intra-herd and intra-host genetic variability of 123 Cryptosporidium parvum isolates was investigated using a multilocus fragment typing approach with eleven variable-number tandem-repeat (VNTR) loci and the GP60 gene. Isolates were collected from intensively farmed diarrheic pre-weaned calves originating from 31 dairy farms in three adjoining regions in northern Spain (País Vasco, Cantabria and Asturias). The multilocus tool demonstrated an acceptable typeability, with 104/123 samples amplifying at all twelve loci. The ML2, TP14, GP60 and the previously un-described minisatellite at locus cgd2_3850 were the most discriminatory markers, while others may be dismissed as monomorphic (MSB) or less informative (CP47, ML1 and the novel minisatellites at loci Cgd1_3670 and Cgd6_3940). The 12-satellite typing tool provided a Hunter-Gaston index (HGDI) of 0.987 (95% CI, 0.982–0.992), and differentiated a total of 70 multilocus subtypes (MLTs). The inclusion of only the four most discriminatory markers dramatically reduced the number of MLTs (n: 44) but hardly reduced the HGDI value. A total of 54 MLTs were distinctive for individual farms, indicating that cryptosporidiosis is an endemic condition on most cattle farms. However, a high rate of mixed infections was detected, suggesting frequent meiotic recombination. Namely, multiple MLTs were seen in most farms where several specimens were analyzed (90.5%), with up to 9 MLTs being found on one farm, and individual specimens with mixed populations being reported on 11/29 farms. Bayesian Structure analysis showed that over 35% of isolates had mixed ancestry and analysis of evolutionary descent using the eBURST algorithm detected a high rate (21.4%) of MLTs appearing as singletons, indicating a high degree of genetic divergence. Linkage analysis found evidence of linkage equilibrium and an overall panmictic structure within the C. parvum population in this discrete geographical area.
000048374 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B82$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2012-32138
000048374 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000048374 590__ $$a2.806$$b2016
000048374 591__ $$aMULTIDISCIPLINARY SCIENCES$$b15 / 63 = 0.238$$c2016$$dQ1$$eT1
000048374 592__ $$a1.236$$b2016
000048374 593__ $$aAgricultural and Biological Sciences (miscellaneous)$$c2016$$dQ1
000048374 593__ $$aMedicine (miscellaneous)$$c2016$$dQ1
000048374 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2016$$dQ1
000048374 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000048374 700__ $$0(orcid)0000-0001-7831-2483$$aQuílez, J$$uUniversidad de Zaragoza
000048374 700__ $$0(orcid)0000-0003-0210-2919$$aMonteagudo, L$$uUniversidad de Zaragoza
000048374 700__ $$0(orcid)0000-0002-9453-7655$$aDel Cacho, E$$uUniversidad de Zaragoza
000048374 700__ $$0(orcid)0000-0002-3532-0384$$aSánchez-Acedo, C$$uUniversidad de Zaragoza
000048374 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000048374 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000048374 773__ $$g11, 2 (2016), 0148811 [16 pp.$$pPLoS One$$tPloS one$$x1932-6203
000048374 8564_ $$s1237572$$uhttps://zaguan.unizar.es/record/48374/files/texto_completo.pdf$$yVersión publicada
000048374 8564_ $$s101477$$uhttps://zaguan.unizar.es/record/48374/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000048374 909CO $$ooai:zaguan.unizar.es:48374$$particulos$$pdriver
000048374 951__ $$a2020-02-21-13:54:05
000048374 980__ $$aARTICLE